Ferrer M, Sanz M L, Prieto I, Oehling A
Department of Allergology and Clinical Immunology, University Clinic Faculty of Medicine, University of Navarra, Pamplona, Spain.
J Investig Allergol Clin Immunol. 1996 Sep-Oct;6(5):271-7.
We studied sulphidoleukotriene (sLT) production, by means of CAST-ELISA (Bühlmann) in 92 atopic (54 pollinic and 38 non-pollinic) patients, and in 9 control subjects, after antigenic stimulation of peripheral blood leukocytes with 20 ng/ml and 2 ng/ml of Lolium perenne pollen extract, in the presence of IL-3. Antigen-specific stimulation of leukocytes from pollinic patients studied during the pollen season led to a sLT production significantly higher (p = 0.03 at 2 ng allergen/ml) than in those studied out of the pollen season. Histamine release was also significantly higher in pollen season than out of the season (p = 0.04 at 20 ng allergen/ml and p < 0.001 at 2 ng allergen/ml). There was a significant positive correlation between sLT production and histamine release (r = 0.67 at 2 ng allergen/ml and r = 0.57 at 20 ng/ml, both p < 0.001), and between sLT production and skin test results (r = 0.5 at 2 ng allergen/ml and r = 0.46 at 20 ng allergen/ml, both p < 0.001). We found that sLT production was lower, although not significantly, in patients older than 40 years, and histamine release was significantly (p = 0.02) higher in women than in men at 2 ng allergen/ml. We conclude that sLT production in pollinic patients is higher when antigenic pressure is increased in the environment, and that sLT quantification by CAST-ELISA might be useful for evaluation of this sensitization, with analogous results to the histamine release test.
我们采用CAST-ELISA（瑞士布尔曼公司）方法，在92例特应性患者（54例花粉症患者和38例非花粉症患者）及9例对照受试者中，于白细胞介素-3存在的情况下，用20纳克/毫升和2纳克/毫升的多年生黑麦草花粉提取物对外周血白细胞进行抗原刺激后，研究了硫代白三烯（sLT）的产生情况。在花粉季节对花粉症患者的白细胞进行抗原特异性刺激后，sLT的产生量显著高于（在2纳克变应原/毫升时p = 0.03）非花粉季节研究的患者。组胺释放量在花粉季节也显著高于非花粉季节（在20纳克变应原/毫升时p = 0.04，在2纳克变应原/毫升时p < 0.001）。sLT产生量与组胺释放量之间存在显著正相关（在2纳克变应原/毫升时r = 0.67，在20纳克/毫升时r = 0.57，两者p均< 0.001），且sLT产生量与皮肤试验结果之间也存在显著正相关（在2纳克变应原/毫升时r = 0.5，在20纳克变应原/毫升时r = 0.46，两者p均< 0.001）。我们发现，40岁以上患者的sLT产生量较低，尽管差异不显著，且在2纳克变应原/毫升时，女性的组胺释放量显著高于男性（p = 0.02）。我们得出结论，当环境中的抗原压力增加时，花粉症患者的sLT产生量会升高，并且通过CAST-ELISA对sLT进行定量分析可能有助于评估这种致敏情况，其结果与组胺释放试验类似。
