Peng H, Ranaldi R, Diss T C, Isaacson P G, Bearzi I, Pan L
Department of Histopathology, University College London Medical School, U.K.
J Pathol. 1998 Aug;185(4):409-12. doi: 10.1002/(SICI)1096-9896(199808)185:4<409::AID-PATH121>3.0.CO;2-T.
A high incidence of Helicobacter pylori infection has been found in patients with gastric MALT (mucosa-associated lymphoid tissue) B-cell lymphoma. Recent studies have indicated that the aggressive strains of the bacterium containing the CagA gene may have direct effects on tumourigenesis. To investigate the involvement of CagA+ strains in MALT lymphomagenesis, a sensitive polymerase chain reaction (PCR)-based detection assay for the gene was developed. DNA extracts from paraffin sections of 123 H. pylori-related gastric biopsies from Italy were analysed, including 56 cases of chronic gastritis, 37 low-grade, and 30 high-grade MALT lymphomas: 30.3 per cent (17/56) of the gastritis cases, 37.8 per cent (14/37) of the low-grade, and 76.7 per cent (23/30) of the high-grade MALT lymphomas were found to contain the CagA gene. The frequency of CagA+ strain infection was significantly higher (P < 0.05) in high-grade than in low-grade MALT lymphoma or gastritis. These results suggest that high-grade gastric MALT lymphoma transformation may be more likely to occur following infection by CagA+ strains of H. pylori.
胃黏膜相关淋巴组织（MALT）B细胞淋巴瘤患者中幽门螺杆菌感染的发生率很高。最近的研究表明，含有CagA基因的侵袭性菌株可能对肿瘤发生有直接影响。为了研究CagA+菌株在MALT淋巴瘤发生中的作用，开发了一种基于聚合酶链反应（PCR）的敏感基因检测方法。对来自意大利的123例幽门螺杆菌相关胃活检组织石蜡切片的DNA提取物进行了分析，包括56例慢性胃炎、37例低级别和30例高级别MALT淋巴瘤：发现30.3%（17/56）的胃炎病例、37.8%（14/37）的低级别和76.7%（23/30）的高级别MALT淋巴瘤含有CagA基因。高级别MALT淋巴瘤中CagA+菌株感染的频率显著高于低级别MALT淋巴瘤或胃炎（P<0.05）。这些结果表明，幽门螺杆菌CagA+菌株感染后，高级别胃MALT淋巴瘤转化可能更易发生。
