Pietropaolo V, Di Taranto C, Degener A M, Jin L, Sinibaldi L, Baiocchini A, Melis M, Orsi N
Institute of Microbiology, University La Sapienza, Rome, Italy.
J Med Virol. 1998 Dec;56(4):372-6. doi: 10.1002/(sici)1096-9071(199812)56:4<372::aid-jmv14>3.0.co;2-4.
The presence of BK virus (BKV) and JC virus (JCV) in autopsy materials (placenta, brain, and kidney) of aborted fetuses was investigated by PCR using two sets of primers, specific for the regulatory region (RR) and for the capsid protein VP1, respectively. The RR of BKV was detected in 12 samples of placenta and brain and in nine samples of kidney obtained from 15 fetuses. Out of the 12 positive cases, four placentas, one brain, and three kidney samples also showed the presence of BKV DNA in the VP1 region. Of 12 placentas from a control group with a normal pregnancy outcome, the RR of BKV was detected in six samples, four of which were also positive for the VP1 region. None of the samples from either group was positive for the RR of JCV. In two cases, the nucleotide sequence of the BK RR demonstrated that the viruses isolated from maternal and fetal tissues showed a high homology with one another and had a characteristic deletion of the R63 box compared to the archetype strain. The results indicate that BKV may be transmitted vertically.
采用两组分别针对调节区（RR）和衣壳蛋白VP1的引物，通过聚合酶链反应（PCR）对流产胎儿尸检材料（胎盘、脑和肾）中的BK病毒（BKV）和JC病毒（JCV）进行检测。在取自15例胎儿的12份胎盘和脑样本以及9份肾样本中检测到BKV的RR。在12例阳性病例中，4份胎盘、1份脑和3份肾样本在VP1区域也显示存在BKV DNA。在妊娠结局正常的对照组的12份胎盘中，6份样本检测到BKV的RR，其中4份在VP1区域也呈阳性。两组的样本均未检测到JCV的RR呈阳性。在两例病例中，BK RR的核苷酸序列表明，从母体和胎儿组织分离出的病毒彼此具有高度同源性，与原型株相比，R63框有特征性缺失。结果表明，BKV可能通过垂直传播。
