De Bortoli M A, Villegas L N, Garraza M H, Gauch M
Universidad Nacional de San Luis (UNSL), Argentina.
Arch Latinoam Nutr. 1998 Jun;48(2):134-40.
Bilateral castration (TX) or sham surgery--respectively--was performed in two groups of twelve male Holtzman rats, 90 days old. Food was available ad libitum to sham-operated rats, and it was restricted to TX ones. After castration, at 30, 60, 90, 120 and 150 days, a segment of the tails was sectioned. At 150 days all rats were subjected to bone mineral densitometry (BMD), sacrificed and the left femurs excised. With the tail's bones (TB) and the femur we determined ash and the concentration of Ca++, PO4(-3), Mg++ and Na+. In the TB, at 30 days the ash and the concentrations of Ca++ and PO4(-3), decreased (p < 0.05) in the TX rats. At 60 days the ash and all analysed ions were reduced (p < 0.05 and p < 0.025) in the TX rats. The decreases were major forward. The more significative decreases in the TB were: in Ca++ at 90 days (p < 0.005); in the ash at 120 days (p < 0.005); in PO4(-3), Mg++ and Na+ at 150 days (p < 0.005). All ions' concentrations and the ash were statistically equal between the TB and the femurs after 150 days of castration. At the same time, the BMD was significantly less (p < 0.05) in the TX rats. The body weigh of the two group was statistically equal during the 150 days. Our results suggest that in 90 days old castrated male rats, the ash and the concentration of calcium, phosphate, magnesium and sodium in the TB are good indicators of bone mass loss, after 30 and 60 days of castration. Besides, 150 days post castration, the ash and the analysed ions are equal in the femur and in the TB. Moreover, the ions' concentrations were better indicators of the bone mass loss than the BMD.
对两组各12只90日龄的雄性霍尔兹曼大鼠分别进行双侧去势（TX）或假手术。假手术大鼠可自由进食，而去势大鼠的食物则受到限制。去势后，在第30、60、90、120和150天，截取一段尾巴。在第150天，对所有大鼠进行骨密度测定（BMD），然后处死并切除左股骨。通过尾巴骨骼（TB）和股骨，我们测定了灰分以及Ca++、PO4(-3)、Mg++和Na+的浓度。在TB中，去势大鼠在第30天时，灰分以及Ca++和PO4(-3)的浓度降低（p < 0.05）。在第60天时，去势大鼠的灰分和所有分析的离子均减少（p < 0.05和p < 0.025）。减少情况在后期更为明显。TB中更显著的减少情况为：第90天时Ca++减少（p < 0.005）；第120天时灰分减少（p < 0.005）；第150天时PO4(-3)、Mg++和Na+减少（p < 0.005）。去势150天后，TB和股骨中所有离子的浓度以及灰分在统计学上相等。同时，去势大鼠的骨密度显著降低（p < 0.05）。两组大鼠的体重在150天内统计学上相等。我们的结果表明，在90日龄去势雄性大鼠中，去势30天和60天后，TB中的灰分以及钙、磷、镁和钠的浓度是骨质流失的良好指标。此外，去势150天后，股骨和TB中的灰分以及分析的离子相等。而且，离子浓度比骨密度更能作为骨质流失的指标。
