[Alcohol dehydrogenase (ADH) in yeast cells. I. Cytoplasmic, mitochondrial and nuclear ADH in Saccharomyces carlsbergensis and Kluyveromyces fragilis].

When grown in a medium containing lactat, Saccharomyces carlsbergensis produces 5 times more ground-plasmatic ADH than Kluyveromyces fragilis. Upon gelectrophoresis, K. fragilis exhibits 7 bands while S. carlsbergensis shows only one. In a polyacrylamid gradient, the ADH's of both strains yield one band, the position of which corresponds to a molecular weight of about 160 000 D. In cell homogenates and mitochondrial fractions of S. carlsbergensis treated with ultrasound, an ADH is detected which exhibits 3 subbands and a molecular weight greater than 1 Megadalton. This ADH does not occur in K. fragilis. Mitochondrial fractions from K. fragilis contain an ADH, the electrophoretical mobility of which is identical to that of the ADH of the groundplasma. Nuclei of S. carlsbergensis also possess ADH whereas those of K. fragilis probably do not.