Tornow R P, Stilling R
Department of Pathophysiology of Vision and Neuro-Ophthalmology, University Eye Hospital, Tübingen, Germany.
Acta Anat (Basel). 1998;162(2-3):163-8. doi: 10.1159/000046482.
To assess the human rod photopigment distribution and sensitivity with high spatial resolution within the central +/-15 degrees and to compare the results of pigment absorption, sensitivity and rod density distribution (number of rods per square degree).
Rod photopigment density distribution was measured with imaging densitometry using a modified Rodenstock scanning laser ophthalmoscope. Dark-adapted sensitivity profiles were measured with green stimuli (17' arc diameter, 1 degrees spacing) using a T ubingen manual perimeter. Sensitivity profiles were plotted on a linear scale and rod photopigment optical density distribution profiles were converted to absorption profiles of the rod photopigment layer.
Both the absorption profile of the rod photopigment and the linear sensitivity profile for green stimuli show a minimum at the foveal center and increase steeply with eccentricity. The variation with eccentricity corresponds to the rod density distribution.
Rod photopigment absorption profiles, retinal sensitivity profiles, and the rod density distribution are linearly related within the central +/-15 degrees. This is in agreement with theoretical considerations. Both methods, imaging retinal densitometry using a scanning laser ophthalmoscope and dark-adapted perimetry with small green stimuli, are useful for assessing the central rod distribution and sensitivity. However, at present, both methods have limitations. Suggestions for improving the reliability of both methods are given.
以高空间分辨率评估中央±15度范围内人眼视杆细胞光色素分布及敏感度，并比较色素吸收、敏感度和视杆细胞密度分布（每平方度视杆细胞数量）的结果。
使用改良的罗敦司得扫描激光检眼镜，通过成像密度测定法测量视杆细胞光色素密度分布。使用图宾根手动视野计，以绿色刺激（17′弧直径，1度间距）测量暗适应敏感度曲线。敏感度曲线以线性比例绘制，视杆细胞光色素光密度分布曲线转换为视杆细胞光色素层的吸收曲线。
视杆细胞光色素的吸收曲线和绿色刺激的线性敏感度曲线在中央凹中心均显示最小值，并随偏心率急剧增加。随偏心率的变化与视杆细胞密度分布相对应。
在中央±15度范围内，视杆细胞光色素吸收曲线、视网膜敏感度曲线和视杆细胞密度分布呈线性相关。这与理论考虑相符。使用扫描激光检眼镜进行视网膜密度成像和使用小的绿色刺激进行暗适应视野检查这两种方法，都有助于评估中央视杆细胞分布和敏感度。然而，目前这两种方法都有局限性。文中给出了提高这两种方法可靠性的建议。
