Okuda K, Murata M, Sugimoto M, Saito Y, Kabasawa Y, Yoshie H, Saku T, Hara K
Department of Periodontology, Niigata University School of Dentistry, Japan.
J Oral Pathol Med. 1998 Nov;27(10):463-9. doi: 10.1111/j.1600-0714.1998.tb01913.x.
The effect of topically applied transforming growth factor beta1 (TGF-beta1) on the rat gingival wound healing process after flap surgery was evaluated by immunohistochemistry for extracellular matrix molecules (ECM), such as tenascin, heparan sulfate proteoglycan (HSPG) and type IV collagen, and for proliferating cell nuclear antigen (PCNA) in fibroblasts. TGF-beta1 solution was applied to the surgical wound experimental sites. Two microg/microl were applied at the time of the operation, and 1 microg/microl at days 1 and 2 after surgery, with contralateral control sites receiving the vehicle alone. Periodontal tissues were histologically examined at 3 and 7 days post-surgery. Tenascin was found to be more strongly stained in the granulation tissue from experimental sites at 3 days post-surgery. At 7 days postsurgery, HSPG-positive areas in granulation tissue had become smaller and there was a prominent proliferation of PCNA-positive fibroblast-like cells and type IV collagen-positive blood vessels. These results suggest that TGF-beta1 applied to surgical wounds influences early proliferation of gingival fibroblast-like cells, the formation of blood vessels, and ECM remodelling. In conclusion, TGF-beta1 application appears to promote granulation tissue formation in periodontal wound healing.
通过免疫组织化学方法，检测细胞外基质分子（ECM），如腱生蛋白、硫酸乙酰肝素蛋白聚糖（HSPG）和IV型胶原，以及成纤维细胞中增殖细胞核抗原（PCNA），评估局部应用转化生长因子β1（TGF-β1）对大鼠翻瓣手术后牙龈伤口愈合过程的影响。将TGF-β1溶液应用于手术伤口实验部位。手术时应用2μg/μl，术后第1天和第2天应用1μg/μl，对侧对照部位仅给予赋形剂。术后3天和7天对牙周组织进行组织学检查。发现术后3天，实验组肉芽组织中腱生蛋白染色更强。术后7天，肉芽组织中HSPG阳性区域变小，PCNA阳性成纤维细胞样细胞和IV型胶原阳性血管显著增殖。这些结果表明，应用于手术伤口的TGF-β1影响牙龈成纤维细胞样细胞的早期增殖、血管形成和ECM重塑。总之，应用TGF-β1似乎能促进牙周伤口愈合中的肉芽组织形成。
