Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran.
Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, 57159-44867, Iran.
Inflammopharmacology. 2019 Jun;27(3):531-537. doi: 10.1007/s10787-018-0510-0. Epub 2018 Jul 6.
This study was conducted to evaluate the effects of the prepared ointments from Mentha piperita essential oil (M. piperita) on wound healing in the infected mice models. Each circular full-thickness wound was inoculated with 25 × 10 units of Staphylococcus aureus and Pseudomonas aeruginosa bacteria strains. The tissue bacterial count, histological analyses and expression levels of IL-10, TNF-α, TGF-β1, IL-1β, CCL2, CXCL1, VEGF and FGF-2 were assessed to identify the different doses of M. piperita on wound healing. Total tissue bacterial count, edema and inflammation level were declined, but the migration of fibroblasts, collagen synthesis and re-epithelization were increased in treated animals with M. piperita. The expression levels of CCL2, CXCL1, IL-1β, TGF-β1 and IL-10 genes were up-regulated in the M. piperita-treated animals compared to the control group. While the expression of TNF-α, VEGF and FGF-2 was down-regulated in comparison to the control group. This study indicated that M. piperita can be used for treatment of the infected wound.
本研究旨在评估胡椒薄荷精油（M. piperita）制备的软膏对感染小鼠模型伤口愈合的影响。每个圆形全层伤口接种 25×10 个金黄色葡萄球菌和铜绿假单胞菌细菌株。评估组织细菌计数、组织学分析以及 IL-10、TNF-α、TGF-β1、IL-1β、CCL2、CXCL1、VEGF 和 FGF-2 的表达水平，以确定 M. piperita 对伤口愈合的不同剂量。M. piperita 处理的动物的总组织细菌计数、水肿和炎症水平下降，但成纤维细胞迁移、胶原合成和再上皮化增加。与对照组相比，M. piperita 处理动物的 CCL2、CXCL1、IL-1β、TGF-β1 和 IL-10 基因的表达水平上调。而与对照组相比，TNF-α、VEGF 和 FGF-2 的表达下调。本研究表明，M. piperita 可用于治疗感染性伤口。
