Chen C T, Chiang H K, Chow S N, Wang C Y, Lee Y S, Tsai J C, Chiang C P
Laser Medicine Research Center, National Taiwan University, Taipei.
J Oral Pathol Med. 1998 Nov;27(10):470-4. doi: 10.1111/j.1600-0714.1998.tb01914.x.
Light-induced fluorescence spectroscopy was conducted on human oral malignant and normal tissues. Under 330-nm excitation wavelength, significant differences in fluorescence intensity were observed around 380- and 460-nm emission. Furthermore, 7,12-dimethylbenz[a]anthracene (DMBA)-induced carcinogenesis in hamster buccal pouch was investigated to elucidate whether similar alterations of fluorescence spectroscopy occurred during the development of squamous cell carcinoma. Similar to the spectral profiles of human oral malignant and normal tissues, the most intense fluorescence peaks in the pouches occurred at 380 nm and 460 nm emission under 330 nm excitation wavelength. At 380 nm emission, the fluorescence intensity of normal pouch mucosa was stronger than those of DMBA-treated abnormal tissues at different stages of carcinogenesis. However, at 460 nm emission, the fluorescence intensity of DMBA-treated tissues was not only stronger than that of normal pouch mucosa but also shifted to 470 nm. These results suggest that under 330 nm excitation wavelength fluorescence spectroscopy may be useful for the detection of oral malignant lesions.
对人体口腔恶性组织和正常组织进行了光诱导荧光光谱分析。在330纳米激发波长下，在380纳米和460纳米发射波长附近观察到荧光强度存在显著差异。此外，研究了7,12-二甲基苯并[a]蒽（DMBA）诱导的仓鼠颊囊癌变，以阐明在鳞状细胞癌发生过程中是否发生类似的荧光光谱变化。与人体口腔恶性组织和正常组织的光谱特征相似，在330纳米激发波长下，颊囊中最强烈的荧光峰出现在380纳米和460纳米发射波长处。在380纳米发射波长下，正常颊囊黏膜的荧光强度在癌变不同阶段均强于DMBA处理的异常组织。然而，在460纳米发射波长下，DMBA处理组织的荧光强度不仅强于正常颊囊黏膜，而且还向470纳米偏移。这些结果表明，在330纳米激发波长下，荧光光谱分析可能有助于口腔恶性病变的检测。
