Farwell D Gregory, Meier Jeremy D, Park Jesung, Sun Yang, Coffman Heather, Poirier Brian, Phipps Jennifer, Tinling Steve, Enepekides Danny J, Marcu Laura
Department of Otolaryngology-Head and Neck Surgery, University of California, Davis Medical Center, Sacramento, CA 95817, USA.
Arch Otolaryngol Head Neck Surg. 2010 Feb;136(2):126-33. doi: 10.1001/archoto.2009.216.
To investigate the benefit of using time-resolved, laser-induced fluorescence spectroscopy for diagnosing malignant and premalignant lesions of the oral cavity.
The carcinogen 7,12-dimethylbenz[a]anthracene (DMBA) was applied to 1 cheek pouch of 19 hamsters. The contralateral pouch and the cheek pouches of 3 hamsters without DMBA exposure served as controls.
University of California, Davis.
Twenty-two golden/Syrian hamsters.
A nitrogen pulse laser was used to induce tissue autofluorescence between the wavelengths of 360 and 650 nm.
Spectral intensities and time-domain measurements were obtained and compared with the histopathologic findings at each corresponding site.
Spectral intensities and lifetime values at 3 spectral bands (SBs; SB1 = 380 +/- 10 nm; SB2 = 460 +/- 10 nm, and SB3 = 635 +/- 10 nm) allowed for discrimination among healthy epithelium, dysplasia, carcinoma in situ, and invasive carcinoma. The lifetime values at SB2 were the most important when distinguishing the lesions using only time-resolved parameters. An algorithm combining spectral fluorescence parameters derived from both spectral and time-domain parameters (peak intensities, average fluorescence lifetimes, and the Laguerre coefficient [zero-order]) for healthy epithelium, dysplasia, carcinoma in situ, and invasive carcinoma provided the best diagnostic discrimination, with 100%, 100%, 69.2%, and 76.5% sensitivity and 100%, 92.2%, 97.1%, and 96.2% specificity, respectively.
The addition of time-resolved fluorescence-derived parameters significantly improves the capability of fluorescence spectroscopy-based diagnostics in the hamster buccal pouch. This technique provides a potential noninvasive diagnostic instrument for head and neck cancer.
研究使用时间分辨激光诱导荧光光谱法诊断口腔恶性和癌前病变的益处。
将致癌物7,12 - 二甲基苯并[a]蒽（DMBA）应用于19只仓鼠的1个颊囊。对侧颊囊以及3只未接触DMBA的仓鼠的颊囊作为对照。
加利福尼亚大学戴维斯分校。
22只金黄/叙利亚仓鼠。
使用氮脉冲激光诱导360至650纳米波长之间的组织自发荧光。
获取光谱强度和时域测量值，并与每个相应部位的组织病理学结果进行比较。
3个光谱带（SBs；SB1 = 380±10纳米；SB2 = 460±10纳米，SB3 = 635±10纳米）的光谱强度和寿命值能够区分健康上皮、发育异常、原位癌和浸润性癌。仅使用时间分辨参数区分病变时，SB2处的寿命值最为重要。一种结合了从光谱和时域参数（峰值强度、平均荧光寿命和拉盖尔系数[零阶]）得出的光谱荧光参数的算法，对健康上皮、发育异常、原位癌和浸润性癌的诊断区分效果最佳，灵敏度分别为100%、100%、69.2%和76.5%，特异性分别为100%、92.2%、97.1%和96.2%。
添加时间分辨荧光衍生参数显著提高了基于荧光光谱法在仓鼠颊囊诊断中的能力。该技术为头颈癌提供了一种潜在的非侵入性诊断工具。
