Bailis J M, Roeder G S
Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06520-8103 USA.
Genes Dev. 1998 Nov 15;12(22):3551-63. doi: 10.1101/gad.12.22.3551.
Development of yeast meiotic chromosome cores into full-length synaptonemal complexes requires the MEK1 gene product, a meiosis-specific protein kinase homolog. The Mek1 protein associates with meiotic chromosomes and colocalizes with the Red1 protein, which is a component of meiotic chromosome cores. Mek1 and Red1 interact physically in meiotic cells, as demonstrated by coimmunoprecipitation and the two-hybrid protein system. Hop1, another protein associated with meiotic chromosome cores, also interacts with Mek1 but only in the presence of Red1. Red1 displays Mek1-dependent phosphorylation, both in vitro and in vivo, and Mek1 kinase activity is necessary for Mek1 function in vivo. Fluorescent in situ hybridization analysis indicates that Mek1-mediated phosphorylation of Red1 is required for meiotic sister-chromatid cohesion, raising the possibility that cohesion is regulated by protein phosphorylation.
酵母减数分裂染色体核心发育成全长联会复合体需要MEK1基因产物，一种减数分裂特异性蛋白激酶同源物。Mek1蛋白与减数分裂染色体相关联，并与Red1蛋白共定位，Red1蛋白是减数分裂染色体核心的一个组成部分。如免疫共沉淀和双杂交蛋白系统所示，Mek1和Red1在减数分裂细胞中发生物理相互作用。Hop1是另一种与减数分裂染色体核心相关的蛋白，也与Mek1相互作用，但仅在有Red1存在时发生。Red1在体外和体内均表现出依赖于Mek1的磷酸化，并且Mek1激酶活性对于Mek1在体内的功能是必需的。荧光原位杂交分析表明，减数分裂姐妹染色单体黏连需要Mek1介导的Red1磷酸化，这增加了黏连受蛋白磷酸化调控的可能性。
