Serre K, Machy P, Grivel J C, Jolly G, Brun N, Barbet J, Leserman L
Centre d'Immunologie de Marseille-Luminy, Marseille, France.
J Immunol. 1998 Dec 1;161(11):6059-67.
To study the relation between the form of an Ag and the response to it, we compared presentation in vitro with hen egg lysozyme (HEL)-specific T cells from TCR transgenic mice of free HEL and liposome-encapsulated HEL by different APC. HEL-specific splenic B cells or bone marrow-derived dendritic cells were incubated with free HEL or HEL-containing liposomes targeted by Ab to either surface Ig, the Fc receptor, or MHC class I and II molecules. Ag presentation by HEL-specific B cells was at least 100-fold more efficient for HEL in surface Ig-targeted liposomes than free HEL taken up by the same receptor or HEL in liposomes targeted to class I or II molecules. Ag presentation by dendritic cells from Fc receptor-targeted vesicles was augmented 1,000-10,000-fold compared with free Ag or nontargeted liposomes, but presentation was also efficient when Ag was targeted to class I or II molecules. These results indicate that Ag-specific B cells and dendritic cells can be equally efficient in stimulating IL-2 production by Ag-specific T cells from unimmunized TCR transgenic mice when the Ag is multivalent and taken up by appropriate receptors. In contrast to B cells, which require engagement of surface Ig for optimal presentation, dendritic cells may present Ag by means of several different cell surface molecules.
为了研究抗原（Ag）的形式与其反应之间的关系，我们通过不同的抗原呈递细胞（APC），比较了游离的溶菌酶（HEL）和脂质体包裹的HEL在体外与来自TCR转基因小鼠的HEL特异性T细胞的呈递情况。将HEL特异性脾B细胞或骨髓来源的树突状细胞与游离的HEL或通过抗体靶向表面Ig、Fc受体或MHC I类和II类分子的含HEL脂质体一起孵育。对于表面Ig靶向脂质体中的HEL，HEL特异性B细胞的抗原呈递效率比相同受体摄取的游离HEL或靶向I类或II类分子的脂质体中的HEL至少高100倍。与游离抗原或非靶向脂质体相比，来自Fc受体靶向囊泡的树突状细胞的抗原呈递增加了1000 - 10000倍，但当抗原靶向I类或II类分子时，呈递也很有效。这些结果表明，当抗原是多价的并被适当的受体摄取时，抗原特异性B细胞和树突状细胞在刺激未免疫的TCR转基因小鼠的抗原特异性T细胞产生白细胞介素-2方面可能同样有效。与需要表面Ig参与以实现最佳呈递的B细胞不同，树突状细胞可能通过几种不同的细胞表面分子呈递抗原。
