Sotníková R, Okruhlicová L, Noskovic P
Institute of Experimental Pharmacology, Slovak Academy of Sciences, Bratislava, Slovakia.
Gen Physiol Biophys. 1998 Sep;17(3):253-64.
The aim of the present study was to evaluate the influence of the antioxidant stobadine on changes in the reactivity of the rat abdominal aorta induced by ischaemia and reperfusion (I/R). In anaesthetized male rats, in vivo ischaemia was elicited by occlusion of the abdominal aorta for 18 hours; reperfusion lasted 30 minutes. The aortal rings were taken from the reperfused portion. Decreased relaxant response to acetylcholine, as a consequence of endothelial injury, was seen after I/R. We also demonstrated I/R-induced reversible ultrastructural changes both in endothelial and smooth muscle cells, predominantly in the mitochondria. Lipid peroxidation was increased in homogenates of I/R aortae; the concentration of thiobarbituric acid reactive substances (TBARS) increased from a control value of 0.97 +/- 0.03 to 2.57 +/- 0.06 nmol/l/mg protein. Stobadine (2 mg/kg i.v., 5 minutes before starting reperfusion) protected the abdominal aorta against I/R-induced decrease of acetylcholine relaxation, and prevented changes in mitochondria and an increase of TBARS concentration. The protective effect of stobadine seems to be due to its antioxidant properties.
本研究的目的是评估抗氧化剂司来吉兰对缺血再灌注（I/R）诱导的大鼠腹主动脉反应性变化的影响。在麻醉的雄性大鼠中，通过阻断腹主动脉18小时诱导体内缺血；再灌注持续30分钟。从再灌注部分获取主动脉环。I/R后可见，由于内皮损伤，对乙酰胆碱的舒张反应降低。我们还证明了I/R在内皮细胞和平滑肌细胞中诱导了可逆的超微结构变化，主要发生在线粒体中。I/R主动脉匀浆中的脂质过氧化增加；硫代巴比妥酸反应性物质（TBARS）的浓度从对照值0.97±0.03增加到2.57±0.06 nmol/l/mg蛋白质。司来吉兰（静脉注射2 mg/kg，在开始再灌注前5分钟）保护腹主动脉免受I/R诱导的乙酰胆碱舒张作用降低，并防止线粒体变化和TBARS浓度增加。司来吉兰的保护作用似乎归因于其抗氧化特性。
