墨水与醋酸，一种用于丛枝菌根真菌的简单染色技术。
Ink and vinegar, a simple staining technique for arbuscular-mycorrhizal fungi.
作者信息
Vierheilig H, Coughlan AP, Wyss U, Piche Y
机构信息
Centre de Recherche en Biologie Forestiere, Universite Laval, Quebec G1K 7P4, Canada.
出版信息
Appl Environ Microbiol. 1998 Dec;64(12):5004-7. doi: 10.1128/AEM.64.12.5004-5007.1998.
Abstract
We developed a reliable, inexpensive, and simple method for staining arbuscular-mycorrhizal fungal colonizations in root tissues. Apart from applications in research, this nontoxic, high-quality staining method also could be of great utility in teaching exercises. After adequate clearing with KOH, an ink-vinegar solution successfully stained all fungal structures, rendering them clearly visible.
摘要
我们开发了一种可靠、廉价且简单的方法来对根组织中的丛枝菌根真菌定殖进行染色。除了在研究中的应用外，这种无毒、高质量的染色方法在教学实践中也可能非常有用。用氢氧化钾充分透明处理后，一种墨水 - 醋溶液成功地对所有真菌结构进行了染色，使其清晰可见。
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