Lavoie J C, Spalinger M, Chessex P
Department of Pediatrics and Research Center Hospital Ste-Justine, University of Montréal, 3175 Chemin Côte Sainte-Catherine, Montréal, Québec H3T 1C5, Canada.
Lung. 1999;177(1):1-7. doi: 10.1007/pl00007623.
Depletion of glutathione, a key antioxidant, accelerates lung injury. Glutathione concentrations are reduced significantly in premature infants with respiratory distress syndrome, leaving them at greater risk of bronchopulmonary dysplasia. A study was designed to verify if the increased glutathione synthetic activity observed in oxygen-dependent and ventilated newborn infants was caused by their postsurgical state. Our objective was to evaluate the role of a general surgical procedure as a factor affecting lung glutathione. One-day-old guinea pig pups, a well characterized animal model for the study of neonatal lung disease, were divided between those undergoing a standardized surgical procedure and those that did not. The pups were fed by their mother. After 4 days the lungs were sampled to determine total glutathione content, activities of gamma-glutamyltranspeptidase, glutathione peroxidase, and reductase as well as the glutathione synthetic activity. The surgical procedure was associated with a specific stimulatory effect limited to glutathione synthetic activity (p < 0.02) leading to an increased (p < 0.02) pulmonary glutathione content. Glutathione concentration was significantly correlated (r2 = 0.67) with the synthetic activity. We concluded that in this animal model an invasive procedure such as a general surgical procedure affects lung glutathione metabolism in a fashion similar to that of hyperoxia. In the lungs, the synthetic activity is a stronger determinant of glutathione concentrations than the activities of the other enzymes involved in maintaining glutathione levels.
谷胱甘肽作为一种关键抗氧化剂，其耗竭会加速肺损伤。患有呼吸窘迫综合征的早产儿体内谷胱甘肽浓度显著降低，这使他们患支气管肺发育不良的风险更高。一项研究旨在验证在依赖氧气和接受通气的新生儿中观察到的谷胱甘肽合成活性增加是否由其术后状态引起。我们的目的是评估普通外科手术作为影响肺谷胱甘肽的一个因素所起的作用。将1日龄豚鼠幼崽（一种用于研究新生儿肺部疾病的特征明确的动物模型）分为接受标准化外科手术的幼崽和未接受手术的幼崽。幼崽由其母亲哺乳。4天后采集肺部样本，以测定总谷胱甘肽含量、γ-谷氨酰转肽酶、谷胱甘肽过氧化物酶和还原酶的活性以及谷胱甘肽合成活性。该外科手术与仅局限于谷胱甘肽合成活性的特定刺激作用相关（p < 0.02），导致肺部谷胱甘肽含量增加（p < 0.02）。谷胱甘肽浓度与合成活性显著相关（r2 = 0.67）。我们得出结论，在这个动物模型中，诸如普通外科手术这样的侵入性操作对肺谷胱甘肽代谢的影响方式与高氧类似。在肺中，合成活性比维持谷胱甘肽水平的其他酶的活性更能决定谷胱甘肽浓度。
