Malighetti V, Arosio M, Bellotti M G, Spadari E, Zambellini Artini M, Spadari F, Riviera L
Istituto di Microbiologia Medica, Università degli Studi, Milano.
Minerva Stomatol. 1998 Sep;47(9):351-9.
The significant frequency of oropharyngeal candidiasis due to C. albicans in HIV-infected and AIDS patients and the undoubted differences in pathogenicity among strains, lead us to study whether a possible correlation exists between the phenotypic characteristics of the fungal strain and the blastospores adhesivity to the human buccal epithelial cells.
From 203 oro-pharyngeal swabs of HIV-infected patients, 133 C. albicans were identified among 159 yeast strains. Analytical strains delineation below the species level was made by the morphotyping method, assigning a morphotype code of three digits, each of which being expressive of one colonial fringe characteristic. To study the possible blastospores adhesivity differences, we have isolated 10 strains of C. albicans, chose according to the more significant colonial morphologies; they were mixed with oral epithelial cells obtained by scraping of 36 HIV-positive patients and 2 volunteer donors HIV-negative at the rate cells/blastospores of 1:20. Suspensions were filtered, fixed and examined by optical microscope (MO) for counting the number of blastospores adhering to 100 epithelial cells and the number of cells with adhering blastospores.
The index obtained by comparing the two qualitative analysis was higher for these isolates producing a rough or very coarse lateral fringes.
This finding suggests that these strains may possess the highest adhesive properties, in fact the index decreases progressively to reach lower values for the strain not producing fringe.
在艾滋病毒感染者和艾滋病患者中，白色念珠菌引起的口咽念珠菌病发生率很高，而且不同菌株在致病性上存在明显差异，这促使我们研究真菌菌株的表型特征与芽生孢子对人颊上皮细胞的黏附性之间是否存在可能的相关性。
从203份艾滋病毒感染患者的口咽拭子中，在159株酵母菌株中鉴定出133株白色念珠菌。通过形态分型法在种以下水平对分析菌株进行划分，赋予一个三位数的形态型代码，每个数字代表一种菌落边缘特征。为了研究芽生孢子黏附性可能存在的差异，我们根据更显著的菌落形态选择并分离出10株白色念珠菌；将它们与通过刮取36名艾滋病毒阳性患者和2名艾滋病毒阴性志愿者的口腔上皮细胞混合，细胞/芽生孢子的比例为1:20。将悬浮液过滤、固定，并用光学显微镜（MO）检查，以计数黏附在100个上皮细胞上的芽生孢子数量以及有芽生孢子黏附的细胞数量。
对于产生粗糙或非常粗糙的侧缘的这些分离株，通过比较两种定性分析获得的指数更高。
这一发现表明这些菌株可能具有最高的黏附特性，实际上，对于不产生边缘的菌株，该指数逐渐降低至较低值。
