Gutiérrez E, Cabeza M
Department of Biological Systems, Metropolitan University-Xochimilco, Mexico, D.F.
Proc West Pharmacol Soc. 1998;41:85-6.
In order to determine the uptake and the metabolism of [3H]T by the mycelium of Penicillium crustosum, cultures of the fungi containing radiolabeled testosterone were developed at different pHs. Also, the metabolism of this androgen in the growth medium of the fungus was determined. Results show that the [3H]T was taken up by the mycelium at pHs 6, 7, 8 and 9. In addition, the presence of [3H]DHT in the incubation medium indicated the participation of 5 alpha-reductase. The maximal activities of this enzyme were determined at pHs 6 and 8, suggesting the presence of two isozymes: type 1 (pH 8), and type 2 (pH 6). Into the mycelium only [3H]T was identified, indicating that isozymes are produced by the fungus under testosterone stimuli.
为了确定[3H]睾酮（[3H]T）被 crustosum青霉的菌丝体摄取及代谢的情况，在不同pH值条件下培养含有放射性标记睾酮的真菌。此外，还测定了这种雄激素在真菌生长培养基中的代谢情况。结果表明，在pH值为6、7、8和9时，菌丝体可摄取[3H]T。此外，孵育培养基中[3H]双氢睾酮（[3H]DHT）的存在表明5α-还原酶参与其中。该酶的最大活性在pH值为6和8时测定，提示存在两种同工酶：1型（pH 8）和2型（pH 6）。在菌丝体中仅鉴定出[3H]T，这表明真菌在睾酮刺激下产生同工酶。
