两种青霉菌（产黄青霉和 crustosum 青霉）将睾酮微生物转化为 5α-二氢睾酮。
Microbial transformations of testosterone to 5alpha-dihydrotestosterone by two species of Penicillium: P. chrysogenum and P. crustosum.
作者信息
Cabeza M S, Gutiérrez E B, García G A, Avalos A H, Hernández M A
机构信息
Department of Biological Systems, Metropolitan University, Xochimilco, Mexico.
出版信息
Steroids. 1999 Jun;64(6):379-84. doi: 10.1016/s0039-128x(98)00115-9.
Two species of Penicillium--P. chrysogenum and P. crustosum--were cultured in presence of [3H]testosterone as a substrate. Both species were shown to reduce the 4,5-double bond in testosterone to give dihydrotestosterone (DHT). The steroids produced were 5alpha-dihydrotestosterone, DHT, 3alpha-hydroxy-5beta-androstan-17-one, 3alpha-hydroy-5alpha-androstan-17-one, 4-androstene-3,17-dione, and 5alpha-androstane-3,17-dione. These products implicate the presence of the 5alpha-reductase, with maximal activity at pH 6 and 8, in both species of Penicillium. The presence of DHT in the growth medium and not in the mycelium suggests that DHT is excreted into the medium.
两种青霉菌——产黄青霉和 crustosum 青霉——在以[3H]睾酮为底物的条件下进行培养。结果表明，这两种青霉菌都能将睾酮中的4,5-双键还原为二氢睾酮（DHT）。所产生的类固醇有5α-二氢睾酮、DHT、3α-羟基-5β-雄甾烷-17-酮、3α-羟基-5α-雄甾烷-17-酮、4-雄烯-3,17-二酮和5α-雄甾烷-3,17-二酮。这些产物表明，在这两种青霉菌中都存在5α-还原酶，其在pH值为6和8时活性最高。生长培养基中存在DHT而菌丝体中不存在，这表明DHT被分泌到了培养基中。
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