Arranz-Peña M L, Tasende-Mata J, Martin-Gil F J
Laboratorio de Análisis Clínicos, Sección de Bioquímica, Hospital Universitario "Del Río Hortega", calle Cardenal Torquemada, s/n, 47010 Valladolid, Spain.
Clin Chem. 1998 Dec;44(12):2499-505.
We report on the performance of four HDL-cholesterol assays: a homogeneous method based on polyethylene glycol-modified enzymes/alpha-cyclodextrin sulfate (PEGME; Kyowa); a homogeneous method based on polyanion-polymer/detergent (PPD; Daiichi); the usual precipitation method with phosphotungstic acid/MgCl2 (PTA); and an ultracentrifugation (UC) procedure. The homogeneous HDL-cholesterol assays (performed with automated analyzers) were precise and correlated well with the PTA and UC assays. The specificity and accuracy of the PEGME method were better than those of the PPD method.
一种基于聚乙二醇修饰酶/硫酸α-环糊精的均相方法（PEGME；协和）；一种基于聚阴离子聚合物/去污剂的均相方法（PPD；第一制药）；常用的磷钨酸/MgCl₂沉淀法（PTA）；以及一种超速离心（UC）程序。均相高密度脂蛋白胆固醇检测方法（使用自动分析仪进行）精确且与PTA和UC检测方法相关性良好。PEGME方法的特异性和准确性优于PPD方法。
