Reno C, Boykiw R, Martinez M L, Hart D A
MaCaig Center for Joint Injury and Arthritis Research, Faculty of Medicine, University of Calgary, Alberta, T2N 4N1, Canada.
Biochem Biophys Res Commun. 1998 Nov 27;252(3):757-63. doi: 10.1006/bbrc.1998.9734.
Wound healing in ligaments is a complex process which leads to functionally impaired scar tissue, even after extended time postinjury. To investigate the potential role of proteinases and inhibitors, as well as potential regulators of their expression, mRNA levels for collagenase, stromelysin, urokinase, PAI-1, and TIMPs 1 to 4 have been assessed by semiquantitative RT-PCR in RNA isolated from rabbit ligaments 3, 6, and 14 weeks postinjury. In addition, mRNA levels for IL-1, TNF, COX-2, and iNOS, potential regulators of proteinase/inhibitor expression, have been assessed. mRNA levels for the proteinases TIMP-1, -2, and -3 and PAI-1 were elevated early in scar tissue, but TIMP-4 mRNA levels exhibited a different pattern. In contrast, mRNA levels for the cytokines iNOS and COX-2 were either unchanged or depressed early after injury. The results indicate that alterations in mRNA levels for proteinases and inhibitors occurring early after injury are likely being influenced by factors other than IL-1, TNF, or products of COX-2 or iNOS.
韧带的伤口愈合是一个复杂的过程，即使在受伤很长时间后，也会导致功能受损的瘢痕组织。为了研究蛋白酶和抑制剂的潜在作用以及它们表达的潜在调节因子，通过半定量逆转录聚合酶链反应（RT-PCR）评估了从受伤后3周、6周和14周的兔韧带中分离出的RNA中胶原酶、基质溶解素、尿激酶、纤溶酶原激活物抑制剂-1（PAI-1）以及基质金属蛋白酶组织抑制剂（TIMPs）1至4的mRNA水平。此外，还评估了白细胞介素-1（IL-1）、肿瘤坏死因子（TNF）、环氧合酶-2（COX-2）和诱导型一氧化氮合酶（iNOS）的mRNA水平，这些是蛋白酶/抑制剂表达的潜在调节因子。瘢痕组织中蛋白酶TIMPs-1、-2和-3以及PAI-1的mRNA水平在早期升高，但TIMP-4的mRNA水平呈现出不同的模式。相比之下，细胞因子iNOS和COX-2的mRNA水平在受伤后早期要么没有变化，要么有所降低。结果表明，受伤后早期蛋白酶和抑制剂mRNA水平的改变可能受到IL-1、TNF或COX-2或iNOS产物以外的因素影响。
