Torii T, Konishi K, Sample J, Takada K
Cancer Institute, Hokkaido University School of Medicine, Sapporo, 060-8638, Japan.
Virology. 1998 Nov 25;251(2):273-8. doi: 10.1006/viro.1998.9411.
The Epstein-Barr virus (EBV) latent membrane protein-1 (LMP-1) gene of the Akata virus strain was cloned, and its nucleotide sequence was determined. Compared with the B95-8 strain, the translation initiation codon for the truncated LMP-1 gene, which is expressed in the lytic cycle, was lost. Immunoblotting showed that Akata EBV produces no truncated LMP-1 protein in any state and that the full-length LMP-1 protein is expressed at a significant level during lytic infection. The results suggest that the truncated LMP-1 protein is dispensable for EBV infection and replication or that the full-length form can "functionally" complement the truncated form if the truncated form has a function.
克隆了赤田病毒株的爱泼斯坦-巴尔病毒（EBV）潜伏膜蛋白1（LMP-1）基因，并测定了其核苷酸序列。与B95-8株相比，在裂解周期中表达的截短型LMP-1基因的翻译起始密码子缺失。免疫印迹显示，赤田EBV在任何状态下均不产生截短型LMP-1蛋白，并且在裂解感染期间全长LMP-1蛋白以显著水平表达。结果表明，截短型LMP-1蛋白对于EBV感染和复制并非必需，或者如果截短型具有功能，全长形式可以在“功能上”补偿截短型。
