钙、锌和硒对镉诱导的大麦根细胞染色体畸变和微核的拮抗作用。

Antagonistic effect of calcium, zinc and selenium against cadmium induced chromosomal aberrations and micronuclei in root cells of Hordeum vulgare.

作者信息

Zhang Y, Xiao H

机构信息

Department of Life Science, Shanxi University, Taiyuan 030006, China.

出版信息

Mutat Res. 1998 Dec 3;420(1-3):1-6. doi: 10.1016/s1383-5718(98)00133-8.

DOI:10.1016/s1383-5718(98)00133-8

PMID:9838018

Abstract

The antagonistic effect of calcium (Ca2+), zinc (Zn2+) and selenium (Se4+) at different concentrations (10-2-10-6 M) against cadmium (Cd2+) induced genotoxic effects in root cells of Hordeum vulgare were studied. The results showed that 10-3-10-5 M could induce chromosomal aberrations and micronuclei formation. But in the treatment with 10-2-10-6 M of Ca2+, Zn2+ and Se4+ together with Cd2+ (10-3-10-5 M), respectively, the frequencies of chromosomal aberrations and micronuclei effectively decreased after 48 h of treatment. The treatment with 10-4-10-6 M of Ca2+ together with 10-4-10-5 M Cd2+, 10-6 M of Zn2+ together with 10-5 M Cd2+ and 10-6 M of Se4+ together with 10-5 M Cd2+ suggested rather obvious antagonistic effects. The order of the antagonisms of Ca2+, Se4+ and Zn2+ against Cd2+ toxicity was Ca2+>Se4+>Zn2+. The degree of antagonisms of Ca2+, Se4+ and Zn2+ against Cd2+ related to their concentration ratio.

摘要

研究了不同浓度（10⁻² - 10⁻⁶ M）的钙（Ca²⁺）、锌（Zn²⁺）和硒（Se⁴⁺）对镉（Cd²⁺）诱导的大麦根细胞遗传毒性效应的拮抗作用。结果表明，10⁻³ - 10⁻⁵ M的镉可诱导染色体畸变和微核形成。但分别用10⁻² - 10⁻⁶ M的Ca²⁺、Zn²⁺和Se⁴⁺与10⁻³ - 10⁻⁵ M的Cd²⁺共同处理后，处理48小时后染色体畸变和微核的频率有效降低。用10⁻⁴ - 10⁻⁶ M的Ca²⁺与10⁻⁴ - 10⁻⁵ M的Cd²⁺、10⁻⁶ M的Zn²⁺与10⁻⁵ M的Cd²⁺以及10⁻⁶ M的Se⁴⁺与10⁻⁵ M的Cd²⁺共同处理显示出相当明显的拮抗作用。Ca²⁺、Se⁴⁺和Zn²⁺对Cd²⁺毒性的拮抗顺序为Ca²⁺>Se⁴⁺>Zn²⁺。Ca²⁺、Se⁴⁺和Zn²⁺对Cd²⁺的拮抗程度与其浓度比有关。