Soto T, Fernández J, Dominguez A, Vicente-Soler J, Cansado J, Gacto M
Departamento de Genética y Microbiología, Facultad de Biología, Universidad de Murcia, 30071 Murcia, Spain.
Biochim Biophys Acta. 1998 Nov 26;1443(1-2):225-9. doi: 10.1016/s0167-4781(98)00214-0.
We have cloned and sequenced the ntp1+ gene that codes for neutral trehalase in the fission yeast Schizosaccharomyces pombe. The ntp1+ gene product (Ntp1p) showed a 45-55% identity with neutral trehalases from other yeasts at the amino acid sequence level. However, in clear contrast to other neutral yeast trehalases so far characterized (which show two cAMP phospho-sites), only one consensus site for cAMP-dependent protein phosphorylation was found in Ntp1p. Northern blot hybridization experiments demonstrated that the Wis-Phh1/Sty1 MAP kinase cascade regulates ntp1+ expression during osmostress.
我们已经克隆并测序了裂殖酵母粟酒裂殖酵母中编码中性海藻糖酶的ntp1⁺基因。ntp1⁺基因产物（Ntp1p）在氨基酸序列水平上与其他酵母的中性海藻糖酶有45%-55%的同一性。然而，与迄今为止所鉴定的其他中性酵母海藻糖酶（具有两个cAMP磷酸化位点）形成鲜明对比的是，在Ntp1p中仅发现了一个cAMP依赖性蛋白磷酸化的共有位点。Northern印迹杂交实验表明，Wis-Phh1/Sty1 MAP激酶级联在渗透胁迫期间调节ntp1⁺的表达。
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