粟酒裂殖酵母中缺乏中性海藻糖酶活性的突变体的特性：对热休克和高盐胁迫的部分保护作用
Characterization of mutants devoid of neutral trehalase activity in the fission yeast Schizosaccharomyces pombe: partial protection from heat shock and high-salt stress.
作者信息
Cansado J, Soto T, Fernandez J, Vicente-Soler J, Gacto M
机构信息
Department of Genetics and Microbiology, Facultad de Biologia, University of Murcia, Spain.
出版信息
J Bacteriol. 1998 Mar;180(5):1342-5. doi: 10.1128/JB.180.5.1342-1345.1998.
Abstract
Exposure of cells of Schizosaccharomyces pombe to heat shock or osmotic upshift results in an increased level of neutral trehalase activity, which is responsible for hydrolysis of intracellular trehalose. We constructed S. pombe mutants lacking neutral trehalase activity by gene replacement at the newly defined ntp1+ locus. Analysis of these mutants revealed that a twofold increase in trehalose accumulation, enhanced acquired thermoresistance, and marked salt tolerance characterized their ability to grow in liquid and solid media. Analysis of the expression of the trehalase gene under heat shock and osmotic upshift revealed the transcriptional activation of ntp1+ in response to both stresses.
摘要
将粟酒裂殖酵母细胞暴露于热休克或渗透压升高环境中会导致中性海藻糖酶活性水平升高，该酶负责细胞内海藻糖的水解。我们通过在新定义的ntp1⁺基因座进行基因替换构建了缺乏中性海藻糖酶活性的粟酒裂殖酵母突变体。对这些突变体的分析表明，海藻糖积累增加两倍、获得性耐热性增强以及显著的耐盐性是它们在液体和固体培养基中生长能力的特征。对热休克和渗透压升高条件下海藻糖酶基因表达的分析揭示了ntp1⁺在两种应激反应下的转录激活。
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