Morelati F, Burlini A, Reis K J, Drago F, Revelli N, Villa M A, Guffanti A, Italiano Z, Parravicini A, Rebulla P, Sirchia G
Centro Trasfusionale e di Immunologia dei Trapianti, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS), Ospedale Maggiore, Milan, Italy.
Transfusion. 1998 Nov-Dec;38(11-12):1037-40. doi: 10.1046/j.1537-2995.1998.38111299056313.x.
Red cell (RBC) phenotyping using column agglutination technology (CAT) is currently limited by the reagents formulated in the system. To overcome this limitation, it was investigated whether monoclonal IgM reagents licensed for use with tube tests produced valid results with CAT.
Commercial CAT, does not contain antisera, was used to evaluate Procedures A (40 microL of reagent and 10 microL of 4% RBCs) and B (50 microL of reagent and 50 microL of 0.8% RBCs) with or without incubation at room temperature. In Study 1, reagents were tested to determine whether potentiators inhibit the passage of antigen-negative RBCs through the column. In Study 2, CAT sensitivity was measured by the use of potency titrations to define a procedure for each reagent that matched or exceeded that of the tube method. In Study 3, the specificity of each reagent was determined in parallel with the CAT and tube tests. Typing of 1644 samples was performed.
Study 1: Free passage was obtained with all reagents. Study 2: Immediate-spin methods using CAT produced the same results as the tube method. Study 3: With 8048 comparisons made, discrepant results were found in 32 transfused patients and in 6 cord blood samples, mainly with Lewis reagents. With comparison of CAT and the standard tube method, complete agreement was obtained with Kell reagents, 99.9-percent agreement with Kidd reagents, and 98.9-percent and 99.4-percent agreement with Lewis reagents.
Most examined reagents seem suitable for use with CAT.
目前，使用柱凝集技术（CAT）进行红细胞（RBC）表型分析受到该系统中所配制试剂的限制。为克服这一限制，研究了用于试管试验的单克隆IgM试剂在CAT中是否能产生有效的结果。
使用不含抗血清的商用CAT来评估程序A（40微升试剂和10微升4%红细胞）和程序B（50微升试剂和50微升0.8%红细胞），在室温下孵育或不孵育。在研究1中，测试试剂以确定增效剂是否会抑制抗原阴性红细胞通过柱体。在研究2中，通过效价滴定测量CAT的灵敏度，以确定每种试剂与试管法匹配或超过试管法的程序。在研究3中，通过CAT和试管试验并行确定每种试剂的特异性。对1644个样本进行了分型。
研究1：所有试剂均能自由通过。研究2：使用CAT的立即离心法产生的结果与试管法相同。研究3：在进行的8048次比较中，在32例输血患者和6例脐血样本中发现了不一致的结果，主要是与Lewis试剂有关。与CAT和标准试管法相比，Kell试剂完全一致，Kidd试剂一致率为99.9%，Lewis试剂一致率为98.9%和99.4%。
大多数检测试剂似乎适用于CAT。
