Yamate J, Tsujino K, Kumagai D, Sato K, Tsukamoto Y, Kuwamura M, Kotani T, Sakuma S, LaMarre J
Department of Veterinary Pathology, College of Agriculture, Osaka Prefecture University, Japan.
J Comp Pathol. 1998 Nov;119(4):443-57. doi: 10.1016/s0021-9975(98)80038-8.
Tumours of uterine smooth muscle are poorly understood neoplasms in which the effects of steroid sex hormones are complex. The influence of progesterone and oestrogen on a transplantable rat uterine smooth muscle tumour line (SMT-Y) was investigated. Female F344 rats given subcutaneous transplants of tumour fragments developed tumours, 1.5-2 cm in diameter, and were then treated with progesterone (10 mg/rat) or 17 beta-oestradiol (50 mg/rat). Tumours in treated groups were compared with those in untreated controls. During a 9-week observation period after treatment, progesterone promoted tumour growth from 4 weeks, with increased numbers of proliferating cells. In contrast, oestradiol inhibited tumour growth from 6 weeks; the degraded tumours, consisting mainly of vacuolated neoplastic cells, had decreased numbers of proliferating cells and increased numbers of apoptotic cells, demonstrable by in-situ terminal deoxyribonucleotide transferase (TdT)-mediated dUTP nick labelling. Immunohistochemically, tumours in control and progesterone groups were labelled positively for alpha-smooth muscle actin (SMA) and desmin but not for vimentin, whereas the degraded tumours in the oestradiol group had reduced reactivity for SMA and desmin but an increased reactivity for vimentin. These results indicate that progesterone may act as a promoter for uterine smooth muscle tumour growth by stimulating mitotic activity, whereas oestrogen may have suppressive effects on tumour growth, accompanied by morphological changes.
子宫平滑肌肿瘤是一类了解甚少的肿瘤，其中类固醇性激素的作用较为复杂。研究了孕酮和雌激素对一种可移植的大鼠子宫平滑肌肿瘤细胞系（SMT-Y）的影响。给雌性F344大鼠皮下移植肿瘤碎片后，它们长出了直径为1.5 - 2厘米的肿瘤，然后用孕酮（10毫克/只大鼠）或17β-雌二醇（50毫克/只大鼠）进行处理。将处理组的肿瘤与未处理的对照组肿瘤进行比较。在处理后的9周观察期内，孕酮从第4周起促进肿瘤生长，增殖细胞数量增加。相比之下，雌二醇从第6周起抑制肿瘤生长；退化的肿瘤主要由空泡化的肿瘤细胞组成，增殖细胞数量减少，凋亡细胞数量增加，这可通过原位末端脱氧核苷酸转移酶（TdT）介导的dUTP缺口末端标记法得以证实。免疫组织化学分析显示，对照组和孕酮组的肿瘤α-平滑肌肌动蛋白（SMA）和结蛋白呈阳性染色，波形蛋白呈阴性染色，而雌二醇组的退化肿瘤对SMA和结蛋白的反应性降低，对波形蛋白的反应性增加。这些结果表明，孕酮可能通过刺激有丝分裂活性而作为子宫平滑肌肿瘤生长的促进剂，而雌激素可能对肿瘤生长具有抑制作用，并伴有形态学改变。
