用于马组织免疫组织化学检测的抗体选择

Antibody selection for immunohistochemical survey of equine tissue.

作者信息

Kalsow C M, Albrecht T R, Steinberg N P, Lunn D P

机构信息

Department of Ophthalmology, University of Rochester School of Medicine and Dentistry, New York 14642, USA.

出版信息

J Comp Pathol. 1998 Nov;119(4):467-72. doi: 10.1016/s0021-9975(98)80040-6.

DOI:10.1016/s0021-9975(98)80040-6

PMID:9839207

Abstract

Immunohistochemical evaluation of equine tissue necessitates the use of antibodies reactive with cells from a heterogeneous population. Lymphoid tissues from 12 horses were fixed in Bouin's fluid, ethanol or formalin and examined for immunohistochemical reactivity with anti-equine and anti-human monoclonal antibodies (MAbs) specific for MHC Class II antigens, T and B lymphocytes, and macrophages. Only a few of the anti-equine MAbs tested were reactive with fixed, paraffin wax-embedded tissue. Anti-human MAbs expanded the desired range of reactivity and increased the consistency in different animals. The anti-equine MAbs conferred species specificity and anti-human MAbs provided an increased spectrum of reactivity.

摘要

马组织的免疫组织化学评估需要使用与异质细胞群体反应的抗体。将12匹马的淋巴组织固定在布安氏液、乙醇或福尔马林中，并用针对MHC II类抗原、T和B淋巴细胞以及巨噬细胞的抗马和抗人单克隆抗体（MAb）检测免疫组织化学反应性。所测试的抗马单克隆抗体中只有少数与固定的石蜡包埋组织发生反应。抗人单克隆抗体扩大了所需的反应范围，并提高了不同动物之间的一致性。抗马单克隆抗体赋予物种特异性，抗人单克隆抗体提供了更广泛的反应谱。