体外梭状芽胞杆菌和脆弱拟杆菌毒素的二八面体蒙脱石中和活性
Dioctahedral smectite neutralization activity of Clostridium difficile and Bacteroides fragilis toxins in vitro.
作者信息
Martirosian G, Rouyan G, Zalewski T, Meisel-Mikołajczyk F
机构信息
Department of Medical Microbiology, Institute of Biostructure Warsaw Medical Academy, Poland.
出版信息
Acta Microbiol Pol. 1998;47(2):177-83.
The neutralization activity of dioctahedral smectite for ten toxigenic Clostridium difficile and eight enterotoxigenic Bacteroides fragilis strains was studied using McCoy and HT 29/C1 cell lines, respectively. Minimalization of the cytopathic effect of C. difficile toxin B on McCoy cell lines by dioctahedral smectite dissolved in PBS was observed. After incubation with dioctahedral smectite the toxic effects of B. fragilis enterotoxins on HT/29C1 (human colon adenocarcinoma cell line) were eliminated. Best neutralization of B. fragilis toxin was achieved using dioctahedral smectite dissolved in BHI.
分别使用 McCoy 细胞系和 HT 29/C1 细胞系研究了二八面体蒙脱石对 10 株产毒素艰难梭菌和 8 株产肠毒素脆弱拟杆菌菌株的中和活性。观察到溶解于 PBS 的二八面体蒙脱石可使艰难梭菌毒素 B 对 McCoy 细胞系的细胞病变效应最小化。与二八面体蒙脱石孵育后，脆弱拟杆菌肠毒素对 HT/29C1（人结肠腺癌细胞系）的毒性作用被消除。使用溶解于脑心浸液（BHI）的二八面体蒙脱石可实现对脆弱拟杆菌毒素的最佳中和。
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