Mermelstein C S, Rodrigues A P, Einicker-Lamas M, Navarrete R E, Farina M, Costa M L
Departamento de Histologia e Embriologia, Universidade Federal do Rio de Janeiro, Brazil.
Tissue Cell. 1998 Oct;30(5):545-53. doi: 10.1016/s0040-8166(98)80035-9.
Euglena gracilis, a unicellular flagellated alga, can display numerous shape changes. These changes are most probably caused by a pellicle and an internal cytoskeleton. In this paper we studied the distribution of the cytoskeletal proteins actin, alpha-actinin, tropomyosin and tubulin in dark-adapted Euglena, using immunofluorescence microscopy. We found that F-actin, alpha-actinin, tropomyosin and tubulin have a distribution that is coincident in the plasma membrane and, in addition, alpha-actinin and tropomyosin are seen in small patches in the cytoplasm, and tubulin in the flagella. We have also studied the distribution of the endoplasmic reticulum, nucleus, and Golgi apparatus of these cells, using fluorescent probes. Both the endoplasmic reticulum and the Golgi apparatus have a meshwork pattern distributed throughout the cytoplasm, and the nucleus has a chromatin evenly distributed in the nucleoplasm.
纤细裸藻是一种单细胞鞭毛藻，能够呈现出多种形态变化。这些变化很可能是由表膜和内部细胞骨架引起的。在本文中，我们使用免疫荧光显微镜研究了暗适应状态下纤细裸藻中细胞骨架蛋白肌动蛋白、α-辅肌动蛋白、原肌球蛋白和微管蛋白的分布。我们发现，F-肌动蛋白、α-辅肌动蛋白、原肌球蛋白和微管蛋白在质膜上的分布是一致的，此外，α-辅肌动蛋白和原肌球蛋白在细胞质中的小斑块中可见，微管蛋白在鞭毛中可见。我们还使用荧光探针研究了这些细胞的内质网、细胞核和高尔基体的分布。内质网和高尔基体都有分布在整个细胞质中的网状模式，细胞核有均匀分布在核质中的染色质。
