Lazarides E
J Cell Biol. 1976 Feb;68(2):202-19. doi: 10.1083/jcb.68.2.202.
During the spreading of a population of rat embryo cells, approximately 40% of the cells develop a strikingly regular network which precedes the formation of the straight actin filament bundles seen in the fully spread out cells. Immunofluorescence studies with antibodies specific for the skeletal muscle structural proteins actin, alpha-actinin, and tropomyosin indicate that this network is composed of foci containing actin and alpha-actinin, connected by tropomyosin-associated actin filaments. Actin filaments, having both tropomyosin and alpha-actinin associated with them, are also seen to extend from the vertices of this network to the edges of the cell. These results demonstrate a specific interaction of alpha-actinin and tropomyosin with actin filaments during the assembly and organization of the actin filament bundles of tissue culture cells. The three-dimensional network they form may be regarded as the structural precursor and the vertices of this network as the organization centers of the ultimately formed actin filament bundles of the fully spread out cells.
在大鼠胚胎细胞群体的铺展过程中，约40%的细胞会形成一个极为规则的网络，该网络先于在完全铺展的细胞中所见的直肌动蛋白丝束的形成。用针对骨骼肌结构蛋白肌动蛋白、α - 辅肌动蛋白和原肌球蛋白的特异性抗体进行的免疫荧光研究表明，这个网络由含有肌动蛋白和α - 辅肌动蛋白的焦点组成，这些焦点由与原肌球蛋白相关的肌动蛋白丝连接。同时含有原肌球蛋白和α - 辅肌动蛋白的肌动蛋白丝也可见从这个网络的顶点延伸到细胞边缘。这些结果表明，在组织培养细胞肌动蛋白丝束的组装和组织过程中，α - 辅肌动蛋白和原肌球蛋白与肌动蛋白丝存在特异性相互作用。它们形成的三维网络可被视为最终形成的完全铺展细胞的肌动蛋白丝束的结构前体，而这个网络的顶点则作为组织中心。
