Shibuya Y, Okabayashi T, Oda K, Tanaka N
First Department of Surgery, Okayama University Medical School, Japan.
Jpn J Clin Oncol. 1998 Oct;28(10):609-14. doi: 10.1093/jjco/28.10.609.
The CD44 is a cell adhesion molecule that is present as numerous isoforms created by mRNA alternative splicing. Expression of variant isoforms of CD44 is associated with tumor growth and metastasis. The aim of this study was to evaluate whether CD44 isoform expression is a prognostic factor in gastric and colorectal cancer.
We performed a polymerase chain reaction analysis following reverse transcriptase treatment for CD44 expression in fresh surgical specimens obtained from 25 colon carcinomas and 30 gastric carcinomas and matched adjacent normal mucosa. We determined the epithelial variant/hematopoietic variant (E/H) ratio (the amount of the CD44 epithelial variant transcript relative to the CD44 hematopoietic variant transcript) in cancer tissues and examined it for correlations with clinicopathological parameters and survival rate.
The E/H ratio in tumor tissue was significantly higher than that in adjacent non-cancerous mucosa. In gastric and colorectal cancer tissues, there was no significant relationship between E/H ratio and clinicopathological parameters. However, patients who died due to tumor recurrence had a higher E/H ratio than surviving patients with colorectal cancer. In gastric cancer, patients with high E/H tumors had a shorter survival time than those with low E/H tumors.
Our results suggest that the E/H ratio is a useful indicator of prognosis in gastric and colorectal carcinoma.
CD44是一种细胞黏附分子，通过mRNA可变剪接产生多种异构体。CD44变异异构体的表达与肿瘤生长和转移相关。本研究旨在评估CD44异构体表达是否为胃癌和结直肠癌的预后因素。
我们对从25例结肠癌和30例胃癌以及匹配的相邻正常黏膜获取的新鲜手术标本进行逆转录酶处理后，进行聚合酶链反应分析以检测CD44表达。我们测定癌组织中的上皮变异体/造血变异体（E/H）比值（CD44上皮变异体转录本相对于CD44造血变异体转录本的量），并检查其与临床病理参数和生存率的相关性。
肿瘤组织中的E/H比值显著高于相邻的非癌黏膜。在胃癌和结直肠癌组织中，E/H比值与临床病理参数之间无显著关系。然而，因肿瘤复发死亡的患者的E/H比值高于结直肠癌存活患者。在胃癌中，E/H比值高的肿瘤患者的生存时间短于E/H比值低的肿瘤患者。
我们的结果表明，E/H比值是胃癌和结直肠癌预后的有用指标。
