Miwa T, Watanabe A, Yamada Y, Shino Y, Yamada T, Yamashita J, Matsuda M, Nakano H
First Department of Surgery, Nara Medical University, Kashihara, Japan.
Cancer. 1996 Jan 1;77(1):25-9. doi: 10.1002/(SICI)1097-0142(19960101)77:1<25::AID-CNCR6>3.0.CO;2-M.
It has been suggested that CD44 splice variants play a role in the progression of certain epithelial cancers and non-Hodgkin's lymphoma. In this study, we investigated the epithelial variant/hematopoietic variant (E/H) ratio (the amount of the CD44 epithelial variant transcript relative to the CD44 hematopoietic variant transcript) in human gastric carcinoma compared with normal gastric mucosa.
The ratio was determined for tumors and adjacent noncancerous mucosa from 30 gastric carcinoma patients using reverse transcription-polymerase chain reaction and Southern blotting. We also determined the tumor (T)E/H--noncancerous mucosa (N)E/H (the difference between E/H ratios of tumor tissue and adjacent noncancerous mucosa) and examined these measurements for correlations with the pathologic features of gastric carcinoma, as well as for their usefulness as an indicator of tumor progression.
The E/H ratio in tumor tissue was significantly higher than in adjacent noncancerous mucosa (P < 0.01). The TE/H--NE/H in patients with lymph node metastases was 0.16 +/- 0.11, compared with 0.07 +/- 0.08 in cases without lymph node metastases (P < 0.05). Significant correlations also were observed between the TE/H--NE/H and the depth of invasion, blood vessel invasion, and lymphatic vessel invasion (P < 0.03, P < 0.03, and P < 0.01, respectively).
Our results suggest that increases in the E/H ratio may be a useful indicator of progression in gastric carcinoma.
有研究表明，CD44剪接变体在某些上皮癌和非霍奇金淋巴瘤的进展中起作用。在本研究中，我们调查了人胃癌与正常胃黏膜中CD44上皮变体/造血变体（E/H）比值（CD44上皮变体转录本相对于CD44造血变体转录本的量）。
使用逆转录聚合酶链反应和Southern印迹法测定了30例胃癌患者肿瘤组织及癌旁非癌黏膜的E/H比值。我们还测定了肿瘤（T）E/H-非癌黏膜（N）E/H（肿瘤组织与癌旁非癌黏膜E/H比值之差），并检查这些测量值与胃癌病理特征的相关性，以及它们作为肿瘤进展指标的有用性。
肿瘤组织中的E/H比值显著高于癌旁非癌黏膜（P < 0.01）。有淋巴结转移患者的TE/H-NE/H为0.16±0.11，无淋巴结转移患者为0.07±0.08（P < 0.05）。TE/H-NE/H与浸润深度、血管浸润和淋巴管浸润之间也存在显著相关性（分别为P < 0.03、P < 0.03和P < 0.01）。
我们的结果表明，E/H比值升高可能是胃癌进展的一个有用指标。
