Wiman A C, Hjertner B, Linné T, Herron B, Allan G, McNeilly F, Adair B, Moreno-López J, Berg M
Department of Veterinary Microbiology, Swedish University of Agricultural Sciences, Biomedical Centre, Uppsala.
J Neurovirol. 1998 Oct;4(5):545-52. doi: 10.3109/13550289809113499.
In order to study persistence of the porcine rubulavirus LPMV, we examined tissue samples collected from pigs 53 days after experimental infection. These pigs survived the initial infection and could clinically be considered to have recovered from the infection. Two of the pigs used in this study were chemically immunosuppressed during the last 4 days before necropsy. No infectious virus or viral antigen could be detected in any tissue using standard methods for virus isolation and detection. However, the presence of viral genomic RNA and mRNA could be demonstrated in the mid brain of the convalescent pig using an optimised RT-nested PCR. Mid brain, forebrain and lung were all shown to contain LPMV RNA in the immunosuppressed convalescent pigs. In addition we examined the P-gene editing in the recovered pigs and conclude that the viral genome is transcriptionally active in these pigs. The relevance of the persistence of LPMV for maintenance and spread within and/or between pig populations is discussed.
为了研究猪腮腺炎病毒（LPMV）的持续性，我们检测了实验感染53天后从猪身上采集的组织样本。这些猪在初次感染中存活下来，临床上可认为已从感染中恢复。本研究中使用的两头猪在尸检前的最后4天接受了化学免疫抑制。使用标准的病毒分离和检测方法，在任何组织中均未检测到传染性病毒或病毒抗原。然而，使用优化的逆转录巢式PCR可在恢复期猪的中脑中证明病毒基因组RNA和mRNA的存在。在免疫抑制的恢复期猪中，中脑、前脑和肺均显示含有LPMV RNA。此外，我们检查了恢复猪中的P基因编辑，并得出结论，病毒基因组在这些猪中具有转录活性。本文讨论了LPMV持续性对于在猪群内和/或猪群间维持和传播的相关性。
