Tsuboi T, Togari A
Department of Pharmacology, School of Dentistry, Aichi-Gakuin University, Nagoya, Japan.
Arch Oral Biol. 1998 Apr;43(4):335-9. doi: 10.1016/s0003-9969(97)00107-6.
The biological activity of carboxyl-terminal parathyroid hormone peptide[53-84] (PTH[53-84]) and aminoterminal peptide [1-34] (PTH[1-34]) during cultivation of the lower molar tooth germ from mouse embryos was examined. PTH[1-34] increased alkaline phosphatase activity in the tooth germ at an early developmental stage (early to advanced bell stage), and decreased it at a late developmental stage (advanced to late bell stage). On the other hand, PTH[53-84] decreased alkaline phosphatase activity at the early stage and increased it at the late stage. Thus, both PTH fragments had distinct effects on tooth development in vitro, which were confirmed by histological observation of their marked effects on the formation of dentine and enamel. These results show that PTH[53-84] has biological activity in the murine tooth germ, opposite to the action of PTH[1-34].
对小鼠胚胎下颌磨牙牙胚培养过程中羧基末端甲状旁腺激素肽[53 - 84](PTH[53 - 84])和氨基末端肽[1 - 34](PTH[1 - 34])的生物活性进行了检测。PTH[1 - 34]在牙胚发育早期（早期至晚期钟状期）增加碱性磷酸酶活性，而在发育后期（晚期至末期钟状期）降低该活性。另一方面，PTH[53 - 84]在早期降低碱性磷酸酶活性，在后期增加该活性。因此，两种PTH片段在体外对牙齿发育均有不同影响，这通过组织学观察其对牙本质和釉质形成的显著作用得到证实。这些结果表明，PTH[53 - 84]在小鼠牙胚中具有生物活性，其作用与PTH[1 - 34]相反。
