Dean G A, Bernales J A, Pedersen N C
Department of Microbiology, Pathology, and Parasitology, College of Veterinary Medicine, North Carolina State University, Raleigh 27606, USA.
Vet Immunol Immunopathol. 1998 Oct 23;65(2-4):125-38. doi: 10.1016/s0165-2427(98)00148-2.
Feline immunodeficiency virus (FIV) is a lentivirus that induces an acquired immunodeficiency in domestic cats. The objective of this study was to compare the immune response of chronically FIV-infected cats and specific pathogen free (SPF) cats to Listeria monocytogenes, a facultative intracellular bacterium. Regional lymph nodes were removed at various times after subcutaneous inoculation with L. monocytogenes and evaluated. Lymph nodes of chronically FIV-infected cats enlarged more slowly and to a lesser degree than SPF cats. This was due to delayed and blunted lymphoid follicle formation and markedly diminished histiocyte influx. The cellular response correlated with a marked upregulation in IL10 transcription and delayed increase in TNF-alpha upregulation in FIV-infected cats. Transcriptional upregulation of IFN-gamma, IL4, and the p40 chain of IL12 was similar in lymph nodes of FIV-infected and SPF cats. Clinically, FIV-infected cats had a more severe response at the site of L. monocytogenes injection and showed signs of systemic bacterial dissemination while SPF cats remained clinically normal. FIV-infected cats generated a delayed hypersensitivity response similar to SPF cats but also had a significantly greater antibody response. Taken together, these data suggest excessive IL10 production may be responsible for the deficiency observed in the innate immune response of chronically FIV-infected cats challenged with L. monocytogenes.
猫免疫缺陷病毒（FIV）是一种慢病毒，可导致家猫获得性免疫缺陷。本研究的目的是比较长期感染FIV的猫和无特定病原体（SPF）猫对兼性细胞内细菌单核细胞增生李斯特菌的免疫反应。在皮下接种单核细胞增生李斯特菌后的不同时间切除局部淋巴结并进行评估。长期感染FIV的猫的淋巴结肿大比SPF猫更慢且程度更低。这是由于淋巴滤泡形成延迟且减弱，以及组织细胞流入明显减少。细胞反应与FIV感染猫中IL10转录的显著上调以及TNF-α上调的延迟增加相关。FIV感染猫和SPF猫的淋巴结中IFN-γ、IL4和IL12的p40链的转录上调相似。临床上，FIV感染猫在单核细胞增生李斯特菌注射部位有更严重的反应，并表现出全身细菌播散的迹象，而SPF猫临床上保持正常。FIV感染猫产生的迟发型超敏反应与SPF猫相似，但抗体反应也明显更强。综上所述，这些数据表明，过量产生的IL10可能是长期感染FIV的猫在受到单核细胞增生李斯特菌攻击时先天免疫反应中观察到的缺陷的原因。
