猫Fas抗原和Fas配体cDNA的分子克隆

Molecular cloning of feline Fas antigen and Fas ligand cDNAs.

作者信息

Mizuno T, Endo Y, Momoi Y, Goto Y, Nishimura Y, Tsubota K, Mikami T, Ohno K, Watari T, Tsujimoto H, Hasegawa A

机构信息

Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan.

出版信息

Vet Immunol Immunopathol. 1998 Oct 23;65(2-4):161-72. doi: 10.1016/s0165-2427(98)00151-2.

DOI:10.1016/s0165-2427(98)00151-2

PMID:9839871

Abstract

The Fas antigen (FasA) and Fas ligand (FasL) are key molecules which mediate apoptosis. For investigation of apoptosis in cats, we isolated molecular clones of feline FasA and FasL cDNAs by using the polymerase chain reaction (PCR) method to amplify cDNAs from feline lymphoma cell lines. These feline FasA and FasL clones contained complete open reading frames encoding 314 and 280 amino acids, respectively. These feline FasA and FasL cDNA clones had structures characteristic of the tumor necrosis factor (TNF) receptor family and TNF family, respectively. The deduced amino acid sequence of feline FasA and feline FasL, respectively showed 45.0%-60.0% and 75.0%-90.0% similarity with their human, mouse and bovine counterparts. These data will be helpful for investigating the role of the FasA and FasL system in apoptosis and for studying the various diseases associated with the deregulation of apoptosis in cats.

摘要

Fas抗原（FasA）和Fas配体（FasL）是介导细胞凋亡的关键分子。为了研究猫的细胞凋亡，我们使用聚合酶链反应（PCR）方法从猫淋巴瘤细胞系中扩增cDNA，从而分离出猫FasA和FasL cDNA的分子克隆。这些猫FasA和FasL克隆包含分别编码314和280个氨基酸的完整开放阅读框。这些猫FasA和FasL cDNA克隆分别具有肿瘤坏死因子（TNF）受体家族和TNF家族的结构特征。猫FasA和猫FasL推导的氨基酸序列分别与其人类、小鼠和牛的对应序列显示出45.0%-60.0%和75.0%-90.0%的相似性。这些数据将有助于研究FasA和FasL系统在细胞凋亡中的作用，并有助于研究与猫细胞凋亡失调相关的各种疾病。