Masumoto T, Ohkubo K, Yamamoto K, Ninomiya T, Abe M, Akbar S M, Michitaka K, Horiike N, Onji M
Third Department of Internal Medicine, Ehime University School of Medicine, Japan.
Hepatogastroenterology. 1998 Sep-Oct;45(23):1630-4.
BACKGROUND/AIMS: Interleukin 8 is known as a chemotactic factor for neutrophils and T cells. Such inflammatory cells are observed in the liver tissue in chronic viral hepatitis. However, it is not known whether interleukin 8 relates to hepatic injury in patients with chronic viral hepatitis. Therefore, we determined serum interleukin 8 levels and identified the cells related to interleukin 8 production in liver tissue.
Studies were performed on 29 patients with chronic viral hepatitis and 20 normal controls. Serum interleukin 8 levels were assayed using a sandwich ELISA. Immunohistochemical examination was performed to identify the cells related to interleukin 8 production by using a rabbit polyvalent antibody to human interleukin 8.
Serum interleukin 8 levels were found to be increased significantly (p<0.05) in patients with chronic viral hepatitis compared with normal controls. They were also increased significantly in patients with chronic active hepatitis compared with chronic persistent hepatitis (p<0.05), and during exacerbation stages compared with remission stages (p<0.05). Out of the 10 patients examined immunohistochemically, interleukin 8 positive cells in the liver tissue were visualized in 7 patients and observed mainly along sinusoids. There was significant correlation between serum interleukin 8 levels and intensity of staining for interleukin 8 in the liver tissue (p<0.05, r=0.869).
Interleukin 8 plays a role in hepatic injury in patients with chronic viral hepatitis, and is mainly produced by nonparenchymal cells in the liver.
背景/目的：白细胞介素8是一种已知的中性粒细胞和T细胞趋化因子。在慢性病毒性肝炎的肝组织中可观察到这类炎症细胞。然而，白细胞介素8是否与慢性病毒性肝炎患者的肝损伤有关尚不清楚。因此，我们测定了血清白细胞介素8水平，并确定了肝组织中与白细胞介素8产生相关的细胞。
对29例慢性病毒性肝炎患者和20例正常对照者进行研究。采用夹心酶联免疫吸附测定法检测血清白细胞介素8水平。通过使用抗人白细胞介素8兔多价抗体进行免疫组织化学检查，以识别与白细胞介素8产生相关的细胞。
发现慢性病毒性肝炎患者的血清白细胞介素8水平较正常对照者显著升高（p<0.05）。与慢性持续性肝炎患者相比，慢性活动性肝炎患者的血清白细胞介素8水平也显著升高（p<0.05），且在病情加重期较缓解期升高（p<0.05）。在接受免疫组织化学检查的10例患者中，7例患者肝组织中可见白细胞介素8阳性细胞，主要沿肝血窦分布。血清白细胞介素8水平与肝组织中白细胞介素8染色强度之间存在显著相关性（p<0.05，r=0.869）。
白细胞介素8在慢性病毒性肝炎患者的肝损伤中起作用，且主要由肝脏中的非实质细胞产生。
