Kuronen P, Volin P, Laitalainen T
Department of Chemistry, University of Helsinki, Finland.
J Chromatogr B Biomed Sci Appl. 1998 Nov 6;718(2):211-24. doi: 10.1016/s0378-4347(98)00351-x.
A multisteroid screening method has been developed based on the use of 1-[4-(2,3-dihydroxypropoxy)phenyl]-1-alkanones as retention index standards and UV absorbance spectra recorded on-line with a diode-array detector using reversed-phase high-performance liquid chromatographic gradient elution with acetonitrile and water. The effect of chromatographic conditions on retention indices of steroids were studied. The method was tentatively applied to profiling of steroids in serum samples.
基于使用1-[4-(2,3-二羟基丙氧基)苯基]-1-烷酮作为保留指数标准品，并采用反相高效液相色谱梯度洗脱（以乙腈和水为流动相），结合二极管阵列检测器在线记录紫外吸收光谱，开发了一种多甾体筛选方法。研究了色谱条件对甾体保留指数的影响。该方法初步应用于血清样品中甾体的分析。
