Elliott S P, Hale K A
Regional Laboratory for Toxicology, City Hospital N.H.S. Teaching Trust, Birmingham, England.
J Anal Toxicol. 1998 Jul-Aug;22(4):279-89. doi: 10.1093/jat/22.4.279.
An analytical database, using high-performance liquid chromatography (HPLC) with diode array ultraviolet (UV) detection, of over 250 toxicologically relevant drugs has been extensively applied to both clinical and forensic toxicology. This general drug screening system, based on a mixed-phase (octadecyl/cyanopropyl) column and gradient reversed-phase HPLC, can identify a wide range of basic, acidic, and neutral drugs and metabolites, some of which are not amenable to gas chromatography and thin-layer chromatography. Compounds are identified using both retention index (RI) value (calculated by interpolation between a series of reference drug markers) and UV spectral data. It has been previously shown that this chromatographic system provides long-term reproducibility and has potentially useful selectivity differences compared with those based on octadecylsilane columns. Development has been undertaken to improve the speed and practicality of the system for emergency toxicology screening by increasing the rate of the elution gradient, while maintaining the applicability of the RI database. The database has also been found to be an important tool in determining optimum strategies for the adaptation of the system for quantitative analyses of drugs and metabolites under isocratic conditions.
一个使用高效液相色谱（HPLC）和二极管阵列紫外（UV）检测的分析数据库，包含250多种毒理学相关药物，已广泛应用于临床和法医毒理学。这个基于混合相（十八烷基/氰丙基）柱和梯度反相HPLC的通用药物筛选系统，可以识别多种碱性、酸性和中性药物及代谢物，其中一些不适用于气相色谱和薄层色谱。使用保留指数（RI）值（通过在一系列参考药物标记物之间插值计算）和紫外光谱数据来鉴定化合物。先前已表明，与基于十八烷基硅烷柱的色谱系统相比，该色谱系统具有长期重现性且具有潜在有用的选择性差异。为提高该系统用于紧急毒理学筛查的速度和实用性，已开展相关研发工作，通过提高洗脱梯度速率，同时保持RI数据库的适用性。该数据库还被发现是确定在等度条件下使该系统适用于药物和代谢物定量分析的最佳策略的重要工具。
