Chikhi-Chorfi N, Pham-Huy C, Galons H, Manuel N, Lowenstein W, Warnet J M, Claude J R
Laboratoire de Toxicologie, Faculté de Pharmacie, Université de Paris V, France.
J Chromatogr B Biomed Sci Appl. 1998 Nov 6;718(2):278-84. doi: 10.1016/s0378-4347(98)00375-2.
A rapid gas-liquid chromatographic assay is developed for the quantification of methadone (Mtd) and its major metabolite, 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP), in biological fluids of opiate addicts. After alkaline extraction from samples with lidocaine hydrochloride as internal standard, Mtd and EDDP are separated on SP-2250 column at 220 degrees C and detected with a thermionic detector. The chromatographic time is about 6 min. The relative standard deviations (R.S.D.) of Mtd and EDDP standards are between 1.5 and 5.5%. Most drugs of abuse (morphine, codeine, narcotine, cocaine, benzoylecgonine, cocaethylene, dextropropoxyphene etc) are shown not to interfere with this technique. The method has been applied to study the levels of Mtd and EDDP metabolite in serum, saliva and urine of patients under maintenance treatment for opiate dependence. EDDP levels were found higher than those of Mtd in urine samples from four treated patients, but lower in serum and undetectable in saliva. However, Mtd concentrations were higher in saliva than in serum.
已开发出一种快速气-液色谱分析法，用于定量测定阿片类成瘾者生物体液中的美沙酮（Mtd）及其主要代谢物2-亚乙基-1,5-二甲基-3,3-二苯基吡咯烷（EDDP）。以盐酸利多卡因为内标从样品中进行碱性萃取后，Mtd和EDDP在220℃下于SP-2250柱上分离，并用热离子检测器检测。色谱时间约为6分钟。Mtd和EDDP标准品的相对标准偏差（R.S.D.）在1.5%至5.5%之间。大多数滥用药物（吗啡、可待因、那可汀、可卡因、苯甲酰爱康宁、古柯乙烯、右丙氧芬等）对该技术无干扰。该方法已应用于研究阿片类药物依赖维持治疗患者血清、唾液和尿液中Mtd和EDDP代谢物的水平。在4例接受治疗患者的尿液样本中，发现EDDP水平高于Mtd，但在血清中较低，在唾液中未检测到。然而，唾液中的Mtd浓度高于血清中的Mtd浓度。
