Pond C M, Mattacks C A
Department of Biology, The Open University, Milton Keynes, UK.
Immunol Lett. 1998 Oct;63(3):159-67. doi: 10.1016/s0165-2478(98)00074-1.
Spontaneous lipolysis in the adipocytes surrounding the popliteal lymph node rose within 1 h of its being activated with a subcutaneous injection of lipopolysaccharide (LPS), reached a peak after 6-9 h, then declined almost to basal levels after 24 h. The response of adipocytes from elsewhere in the same depot was delayed and smaller. Following the simulated immune challenge, perinodal adipocytes were consistently more sensitive to noradrenalin at 10(-8) and 10(-7) M than those elsewhere in the same depot, but the maximum lipolysis, in the presence of 10(-5) M noradrenalin, was similar in all popliteal samples. These effects were increased by incubating adipose tissue explants for 24 h in tissue culture medium, suggesting autocrine amplification of the initial stimuli. Incubation with interleukin-4 (IL-4, 10 ng/ml) abolished the increase in lipolysis in samples around the activated lymph node and depressed it to below control values in other adipocytes. In vivo stimulation of the popliteal node increased maximum lipolysis in the presence of 10(-5) M noradrenalin in samples from around mesenteric lymph nodes and after 24 h incubation, in omental perinodal adipocytes. No effects of any pre-treatments were detected in perirenal adipocytes. We conclude that the adipocytes surrounding lymph nodes are actively involved in local, transient immune responses. Their participation may explain why most major lymph nodes are embedded in adipose tissue.
用皮下注射脂多糖（LPS）激活腘淋巴结周围的脂肪细胞后，其自发脂解在1小时内升高，6 - 9小时后达到峰值，然后在24小时后几乎降至基础水平。同一脂肪库其他部位的脂肪细胞反应延迟且程度较小。模拟免疫刺激后，淋巴结周围的脂肪细胞在10^(-8)和10^(-7) M去甲肾上腺素作用下始终比同一脂肪库其他部位的脂肪细胞更敏感，但在10^(-5) M去甲肾上腺素存在下的最大脂解在所有腘部样本中相似。通过在组织培养基中培养脂肪组织外植体24小时，这些效应增强，提示初始刺激的自分泌放大。用白细胞介素 - 4（IL - 4，10 ng/ml）孵育可消除激活淋巴结周围样本中脂解的增加，并使其他脂肪细胞中的脂解降低至对照值以下。体内刺激腘淋巴结可增加肠系膜淋巴结周围样本在10^(-5) M去甲肾上腺素存在下以及网膜淋巴结周围脂肪细胞在孵育24小时后的最大脂解。在肾周脂肪细胞中未检测到任何预处理的影响。我们得出结论，淋巴结周围的脂肪细胞积极参与局部短暂免疫反应。它们的参与可能解释了为什么大多数主要淋巴结都嵌入脂肪组织中。
