Mora D, Parini C, Fortina M G, Manachini P L
Department of Food Science and Microbiology, Industrial Microbiology Section, University of Milano, Italy.
Syst Appl Microbiol. 1998 Aug;21(3):454-60. doi: 10.1016/S0723-2020(98)80056-8.
Pediococcus acidilactici, Pediococcus parvulus and Lactobacillus plantarum pediocin AcH/PA-1 produces strains were studied with the aim to investigate their common genetic pediocin determinant using pedA, pedB, pedC and pedD gene-targeted PCR assay. Single Strand Conformation Polymorphism and restriction analysis of pedA and pedC amplified fragments from the three different species did not show any differences in sequence while these analysis carried out on pedB and pedD amplified fragments highlighted differences related to the three species analyzed harboring these plasmid encoded genes. Furthermore different multiplex PCR assay using IdhD, pedA and pedD as target genes were developed to clearly identify the pediocin AcH/PA-1 producer strains and to obtain the simultaneous identification of the P. acidilactici strains.
研究了嗜酸片球菌、微小片球菌和植物乳杆菌中产生片球菌素AcH/PA-1的菌株，目的是使用靶向pedA、pedB、pedC和pedD基因的PCR检测法研究它们共同的片球菌素决定基因。对这三个不同物种的pedA和pedC扩增片段进行单链构象多态性和限制性分析，未发现序列有任何差异，而对pedB和pedD扩增片段进行的这些分析则突出了与携带这些质粒编码基因的三个分析物种相关的差异。此外，还开发了以IdhD、pedA和pedD为靶基因的不同多重PCR检测法，以明确鉴定片球菌素AcH/PA-1产生菌株，并同时鉴定嗜酸片球菌菌株。
