Food Microbiology Department, CSIR-Central Food Technological Research Institute, Mysore, 570020, India.
J Basic Microbiol. 2013 Jul;53(7):555-61. doi: 10.1002/jobm.201200079. Epub 2012 Aug 23.
To evaluate the presence of mobile genetic elements (MGEs) in intergeneric and interspecific pediocin producing lactic acid bacteria (LAB) the flanking regions of the pediocin PA-1/AcH (pediocin PA-1) operon was characterized. In Enterococcus faecium Acr4 and Lactobacillus plantarum Acr2 a variation in the amplicon size in the downstream region of the operon was identified, suggesting a deletion in this region. Beyond that, in pediocin PA-1 encoding plasmids MGEs such as ISLpl1 and mobilization regions were detected by Southern hybridization analysis. Phylogenetic analyses of the E. faecium Acr4 ISLpl1 gene sequence suggested the gene transfer from lactobacilli in the environment. The tyrosine recombinase detected in pediocin plasmids of Pediococcus acidilactici H and K7 indicate a possible transfer of the entire operon among LAB. Since these elements are known to be associated with transfer of genes linked to the bacteriocin production, antibiotic resistance, and sugar utilization, we suggest similar mechanism for natural spread of pediocin PA-1 operon among different bacterial species.
为了评估移动遗传元件（MGEs）在种间和属间肠球菌素产生乳酸菌（LAB）中的存在，我们对肠球菌素 PA-1/AcH（肠球菌素 PA-1）操纵子的侧翼区域进行了特征分析。在屎肠球菌 Acr4 和植物乳杆菌 Acr2 中，发现了操纵子下游区域的扩增子大小发生了变化，表明该区域发生了缺失。除此之外，在肠球菌素 PA-1 编码质粒中，通过 Southern 杂交分析检测到了 MGEs，如 ISLpl1 和移动区域。屎肠球菌 Acr4 ISLpl1 基因序列的系统发育分析表明，该基因是从环境中的乳杆菌中转移而来的。在戊糖片球菌 H 和 K7 的肠球菌素质粒中检测到的酪氨酸重组酶表明，整个操纵子可能在 LAB 之间发生了转移。由于这些元件已知与与细菌素产生、抗生素抗性和糖利用相关的基因转移有关，我们建议肠球菌素 PA-1 操纵子在不同细菌物种之间自然传播的机制类似。
