Witko-Sarsat V, Halbwachs-Mecarelli L, Sermet-Gaudelus I, Bessou G, Lenoir G, Allen R C, Descamps-Latscha B
INSERM U90, HOPITAL nECKER, 161 RUE DE Sévres, 75015 Paris, France.
J Infect Dis. 1999 Jan;179(1):151-62. doi: 10.1086/314532.
Blood phagocyte opsonin receptor CR1 (CD35) and CR3 (CD11b) functions were examined in cystic fibrosis (CF) patients with endobronchial Staphylococcus aureus or Pseudomonas aeruginosa chronic infection, CF patients without infection, heterozygous, non-CF patients with chronic pulmonary infection, and healthy controls. Circulating and platelet-activating factor (PAF)-primed phagocyte luminol luminescence responses to complement-opsonized zymosan were increased in both groups of infected CF and non-CF children relative to uninfected CF children and healthy control children and adults. The ratio between circulating and PAF-primed phagocyte responses was significantly elevated in all children with CF, and in these, the ratio could serve as an indicator of response to antibiotic treatment. The ratios of circulating and PAF-primed phenotypic expression for CR1, CR3, and FcgammaRIII (CD16), but not FcgammaRII (CD32), correlated with the functional ratios. Phagocyte opsonin receptor response capacity might be used for evaluation of inflammation and infection in CF patients.
在患有支气管内金黄色葡萄球菌或铜绿假单胞菌慢性感染的囊性纤维化（CF）患者、未感染的CF患者、杂合子非CF慢性肺部感染患者以及健康对照中，检测了血液吞噬细胞调理素受体CR1（CD35）和CR3（CD11b）的功能。相对于未感染的CF儿童以及健康对照儿童和成人，感染的CF儿童和非CF儿童两组中，循环及血小板活化因子（PAF）预刺激的吞噬细胞对补体调理酵母聚糖的鲁米诺发光反应均增强。所有CF儿童中，循环及PAF预刺激的吞噬细胞反应之比显著升高，并且在这些儿童中，该比值可作为对抗生素治疗反应的指标。CR1、CR3和FcγRIII（CD16）而非FcγRII（CD32）的循环及PAF预刺激表型表达之比与功能比值相关。吞噬细胞调理素受体反应能力可用于评估CF患者的炎症和感染情况。
