Bodini Alessandro, D'Orazio Ciro, Peroni Diego, Corradi Massimo, Folesani Giuseppina, Baraldi Eugenio, Assael Barouk M, Boner Attilio, Piacentini Giorgio L
Department of Pediatrics, University of Verona, Verona, Italy.
Pediatr Pulmonol. 2005 Dec;40(6):494-9. doi: 10.1002/ppul.20336.
Leukotriene B(4) (LTB(4)) and interleukin-8 (IL-8) are inflammatory mediators involved in the neutrophil response to pulmonary bacterial colonization in cystic fibrosis (CF). The aim of this study was to investigate whether the LTB(4) and IL-8 levels in exhaled breath condensate (EBC) could be related to the type of bacterial colonization in CF patients. The pH level in EBC was analyzed as an estimate of airway acidification. Forty children were evaluated: 10 CF patients with P. aeruginosa, 10 CF patients with S. aureus, 10 not colonized CF patients, and 10 healthy children. LTB(4) and IL-8 in EBC were analyzed by specific enzyme immunoassay kits (EIA). The pH of EBC was measured with a pH-meter after deareation by bubbling with argon. Exhaled LTB(4) was higher in CF children with P. aeruginosa compared to those with S. aureus (P < 0.01), not colonized (P < 0.001), and healthy children (P < 0.01). Exhaled IL-8 was elevated in CF patients colonized by P. aeruginosa compared with other subgroups (vs. not colonized, P < 0.05; vs. healthy children, P < 0.001). IL-8 levels were higher in CF children with S. aureus than in healthy children (P < 0.05). There was an increase in IL-8 levels in not colonized CF patients compared with healthy children (P < 0.05). EBC pH was higher in healthy children compared to CF patients not colonized (P < 0.05). Our data suggest that EBC is suitable for evaluating neutrophil inflammatory mediators (LTB(4), IL-8, and pH) involved in the response to pulmonary bacterial colonization in CF children.
白三烯B4（LTB4）和白细胞介素-8（IL-8）是参与囊性纤维化（CF）患者肺部细菌定植时中性粒细胞反应的炎症介质。本研究的目的是调查呼出气体冷凝液（EBC）中的LTB4和IL-8水平是否与CF患者的细菌定植类型有关。分析EBC中的pH水平以评估气道酸化情况。对40名儿童进行了评估：10名感染铜绿假单胞菌的CF患者、10名感染金黄色葡萄球菌的CF患者、10名未发生细菌定植的CF患者和10名健康儿童。通过特定的酶免疫分析试剂盒（EIA）分析EBC中的LTB4和IL-8。用pH计在通过氩气鼓泡除气后测量EBC的pH。与感染金黄色葡萄球菌的CF儿童相比，感染铜绿假单胞菌的CF儿童呼出的LTB4更高（P<0.01），与未发生细菌定植的CF儿童相比（P<0.001），与健康儿童相比（P<0.01）。与其他亚组相比，感染铜绿假单胞菌的CF患者呼出的IL-8升高（与未发生细菌定植的CF患者相比，P<0.05；与健康儿童相比，P<0.001）。感染金黄色葡萄球菌的CF儿童的IL-8水平高于健康儿童（P<0.05）。与健康儿童相比，未发生细菌定植的CF患者的IL-8水平有所升高（P<0.05）。与未发生细菌定植的CF患者相比，健康儿童的EBC pH更高（P<0.05）。我们的数据表明，EBC适用于评估CF儿童肺部细菌定植反应中涉及的中性粒细胞炎症介质（LTB4、IL-8和pH）。
