Berry J D, Peeling R W, Brunham R C
Department of Medical Microbiology, University of Manitoba, Winnipeg, Canada.
J Infect Dis. 1999 Jan;179(1):180-6. doi: 10.1086/314538.
The anamnestic antibody response to the Chlamydia trachomatis major outer membrane protein (MOMP) was evaluated in mice after priming with serovar C and boosting either with the homologous serovar or with heterologous serovars (A, H, K, and B). Microimmunofluorescence antibody responses demonstrated that boosting with heterologous serovars strongly recalled antibody to serovar C, typical of an original antigenic sin (OAS) response. Boosting with serovars antigenically related to serovar C (A, H, and K) recalled antibody to the variable domain 1 (VD1) peptide of the MOMP of serovar C as determined by a pin-peptide ELISA. Complete amino acid substitution analysis of the VD1 peptide epitope of the MOMP showed that the original antigenic sin response to each boosting serovar contained antibodies with unique patterns of VD1 peptide recognition. The data suggest that antigenically related C. trachomatis serovars differentially recruit B cell lineages from a heterogeneous memory B cell pool that had been induced by priming with the original serovar and thus account for the OAS antibody response.
在用血清型C致敏小鼠后，再用同源血清型或异源血清型（A、H、K和B）进行加强免疫，评估小鼠对沙眼衣原体主要外膜蛋白（MOMP）的回忆性抗体反应。微量免疫荧光抗体反应表明，用异源血清型加强免疫能强烈唤起针对血清型C的抗体，这是典型的原始抗原罪（OAS）反应。通过针肽ELISA测定，用与血清型C抗原相关的血清型（A、H和K）加强免疫能唤起针对血清型C的MOMP可变区1（VD1）肽的抗体。对MOMP的VD1肽表位进行的全氨基酸替换分析表明，对每种加强免疫血清型的原始抗原罪反应都包含具有独特VD1肽识别模式的抗体。数据表明，抗原相关的沙眼衣原体血清型从由原始血清型致敏诱导产生的异质性记忆B细胞库中差异性地募集B细胞谱系，从而导致了OAS抗体反应。
