Elliott J J, Ruble D L, Zaucha G M, Jaax G P, Waag D M
Veterinary Medicine Division, United States Army Medical Research Institute of Infectious Diseases (USAMRIID), Fort Detrick, Frederick, MD, USA.
Acta Virol. 1998 Jun;42(3):147-55.
Coxiella burnetii phase I whole cell vaccine (WCV) is associated with risk of severe local delayed-type hypersensitivity (DTH) reactions in previously immunized individuals or those sensitized by natural exposure. We compared this vaccine to another investigational vaccine derived by chloroform-methanol extraction of phase I whole cells (chloroform-methanol residue vaccine, CMRV). Hairless guinea pigs, sensitized with either WCV or CMRV, were given 60,600 and 6,000 ng of WCV or CMRV in an intradermal (i.d.) skin test. The i.d. administration of WCV consistently caused more host reactions than comparable doses of CMRV in guinea pigs sensitized with either WCW or CMRV, suggesting that CMRV may be a safer vaccine. However, the CMRV was not innocuous and caused significant indurated lesions and micro-abscesses at the 600 ng and 6,000 ng skin test sites.
伯纳特柯克斯体I相全细胞疫苗（WCV）与既往免疫个体或自然暴露致敏个体发生严重局部迟发型超敏反应（DTH）的风险相关。我们将这种疫苗与另一种通过氯仿-甲醇提取I相全细胞得到的研究性疫苗（氯仿-甲醇残留疫苗，CMRV）进行了比较。用WCV或CMRV致敏的无毛豚鼠，在皮内（i.d.）皮肤试验中分别给予60,600 ng和6,000 ng的WCV或CMRV。在分别用WCW或CMRV致敏的豚鼠中，皮内注射WCV始终比同等剂量的CMRV引起更多的宿主反应，这表明CMRV可能是一种更安全的疫苗。然而，CMRV并非无害，在600 ng和6,000 ng皮肤试验部位引起了明显的硬结病变和微脓肿。
