Ruble D L, Elliott J J, Waag D M, Jaax G P
United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, MD 21701.
Lab Anim Sci. 1994 Dec;44(6):608-12.
The previously established model for determining dermal granulomatous reactivity of Q fever vaccines uses footpad injections to sensitize Hartley guinea pigs. We describe a substantial refinement to that model by substituting an alternate injection route and replacing the model with the hairless Hartley guinea pig. The experimental design incorporates a matrix of various antigen and adjuvant combinations administered by subcutaneous (SC) and intradermal routes of administration. The two Coxiella burnetii antigens evaluated were phase-I whole-cell vaccine (WCV) and phase-I chloroform-methanol residue vaccine. The adjuvants used were complete Freund's (CFA), incomplete Freund's, and RIBI's De-Tox. Phase-I C. burnetii WCV in CFA administered SC provided the most efficacious regimen evaluated for sensitizing hairless guinea pigs. The establishment of this animal model allows evaluation of candidate Q fever vaccines in a more humane model than that previously available.
先前建立的用于确定Q热疫苗皮肤肉芽肿反应性的模型，采用足垫注射法使Hartley豚鼠致敏。我们通过改用另一种注射途径，并将模型替换为无毛Hartley豚鼠，对该模型进行了大幅改进。实验设计纳入了通过皮下（SC）和皮内给药途径给予的各种抗原和佐剂组合的矩阵。评估的两种伯氏考克斯体抗原为I相全细胞疫苗（WCV）和I相氯仿-甲醇残留疫苗。使用的佐剂有完全弗氏佐剂（CFA）、不完全弗氏佐剂和RIBI公司的去毒剂。皮下注射给予CFA中的I相伯氏考克斯体WCV，是评估的使无毛豚鼠致敏的最有效方案。该动物模型的建立，使得能够在比以前更人道的模型中评估候选Q热疫苗。
