Borgfeldt C, Casslén B, Liu C L, Hansson S, Lecander I, Astedt B
Department of Obstetrics and Gynecology, University Hospital, Lund, Sweden.
Int J Cancer. 1998 Dec 18;79(6):588-95. doi: 10.1002/(sici)1097-0215(19981218)79:6<588::aid-ijc6>3.0.co;2-w.
Urokinase plasminogen activator (u-PA) plays a pivotal role in tissue degradation during tumor spread and metastasis. We have quantitated u-PA in tissue homogenates of 31 serous ovarian tumors and localized u-PA and its mRNA in tissue sections of 26 serous ovarian tumors. The content of u-PA was higher in malignant than in benign tumors, with the highest levels being found in poorly differentiated cancers. In tissue sections, the u-PA mRNA was hybridized with a radiolabeled RNA probe. Signals were almost exclusively found in the epithelium in benign and borderline tumors and in well-differentiated cancers. Poorly differentiated tumors and metastases exhibited prominent stromal expression of u-PA mRNA, whereas epithelial expression was weak or absent. Immuno-histochemical staining co-localized u-PA antigen with its mRNA in the epithelium of benign and borderline tumors and in well-differentiated cancers. Poorly differentiated malignant tumors showed extensive immunostaining in the epithelium in addition to stromal staining. The u-PA mRNA-expressing and u-PA-immunostained cells in the stroma were not tumor cells since no cells in the stroma were positive for cytokeratin. Poorly differentiated tumors had increased numbers of stromal macrophages (CD68), and they co-localized with some of the u-PA-positive cells. The presence of u-PA antigen and the absence of u-PA mRNA in tumor epithelium of poorly differentiated tumors and metastases together with the presence of u-PA mRNA in the stroma suggests production in stromal cells and subsequent binding to receptor sites in tumor cells.
尿激酶型纤溶酶原激活剂（u-PA）在肿瘤扩散和转移过程中的组织降解中起关键作用。我们对31例浆液性卵巢肿瘤组织匀浆中的u-PA进行了定量，并对26例浆液性卵巢肿瘤组织切片中的u-PA及其mRNA进行了定位。恶性肿瘤中u-PA的含量高于良性肿瘤，在低分化癌中含量最高。在组织切片中，u-PA mRNA与放射性标记的RNA探针杂交。在良性和交界性肿瘤以及高分化癌中，信号几乎仅在上皮细胞中发现。低分化肿瘤和转移灶中u-PA mRNA在基质中表达显著，而上皮表达较弱或缺失。免疫组织化学染色显示，在良性和交界性肿瘤以及高分化癌的上皮细胞中，u-PA抗原与其mRNA共定位。低分化恶性肿瘤除基质染色外，上皮细胞也有广泛的免疫染色。基质中表达u-PA mRNA和u-PA免疫染色的细胞不是肿瘤细胞，因为基质中没有细胞角蛋白阳性的细胞。低分化肿瘤基质中的巨噬细胞（CD68）数量增加，且它们与一些u-PA阳性细胞共定位。低分化肿瘤和转移灶的肿瘤上皮细胞中存在u-PA抗原但不存在u-PA mRNA，同时基质中存在u-PA mRNA，这表明u-PA是由基质细胞产生并随后与肿瘤细胞中的受体位点结合。
