O'neill D F, Powell J F, Standen E M, Youson J H, Warby C M, Sherwood N M
Department of Biology, University of Victoria, Victoria, British Columbia, V8W 2Y2.
Gen Comp Endocrinol. 1998 Dec;112(3):415-25. doi: 10.1006/gcen.1998.7163.
The molecular forms of gonadotropin-releasing hormone (GnRH) were examined in the bonytongue fishes (Osteoglossomorpha), one of the most ancient living teleost groups. These fish represent a phylogenetic link between the early ray-finned fishes and the modern teleosts. Five representative species from four of six bonytongue families were examined for GnRH using high-performance liquid chromatography and radioimmunoassay techniques with antisera raised against salmon (s), chicken-II (c-II), and mammalian (m) forms of GnRH. Salmon GnRH and cGnRH-II were identified in four of the species (arawana, elephantnose, false featherfin, Asiatic featherfin) whereas in the butterfly fish, mGnRH and cGnRH-II were identified. Our data suggest that teleosts such as eels and butterfly fish, which have mGnRH like that of even earlier ray-finned fishes, may have evolved before fish with sGnRH. We also suggest that sGnRH first appeared in the Osteoglossomorpha. The phylogenetic relationship of the eels (Anguillidae), butterfly fish (Pantodontidae), and bonytongue fish among other teleosts needs to be reexamined using additional characteristics.
在骨舌鱼（骨舌鱼目）中检测了促性腺激素释放激素（GnRH）的分子形式，骨舌鱼是现存最古老的硬骨鱼群之一。这些鱼类代表了早期辐鳍鱼和现代硬骨鱼之间的系统发育联系。使用高效液相色谱法和放射免疫测定技术，用针对鲑鱼（s）、鸡-II（c-II）和哺乳动物（m）形式的GnRH的抗血清，对六个骨舌鱼科中四个科的五个代表性物种进行了GnRH检测。在四个物种（龙鱼、象鼻鱼、假鳍羽鱼、亚洲鳍羽鱼）中鉴定出了鲑鱼GnRH和cGnRH-II，而在蝴蝶鱼中鉴定出了mGnRH和cGnRH-II。我们的数据表明，像鳗鱼和蝴蝶鱼这样具有与更早的辐鳍鱼相似的mGnRH的硬骨鱼，可能在具有sGnRH的鱼类之前就已经进化了。我们还认为sGnRH首先出现在骨舌鱼目中。需要利用其他特征重新审视鳗鱼（鳗鲡科）、蝴蝶鱼（齿蝶鱼科）和骨舌鱼与其他硬骨鱼之间的系统发育关系。
